Fermentation performance and physiology of two strains of Saccharomyces cerevisiae during growth in high gravity spruce hydrolysate and spent sulphite liquor

BMC Biotechnology

Emma Johansson²  Charilaos Xiros¹  Christer Larsson¹ 

[1] Department of Chemical and Biological Engineering, Chalmers University of Technology, 412 96 Göteborg, Sweden

[2] SP Processum AB, 891 22 Örnsköldsvik, Sweden

关键词: High gravity fermentation;    Fermentation capacity;    Energy charge;    Nutrients;    Lignocellulosic material;   

Others  :  834843
DOI  :  10.1186/1472-6750-14-47

 received in 2013-12-12, accepted in 2014-04-24,  发布年份 2014

【 摘 要 】

Background

Lignocellulosic materials are a diverse group of substrates that are generally scarce in nutrients, which compromises the tolerance and fermentation performance of the fermenting organism. The problem is exacerbated by harsh pre-treatment, which introduces sugars and substances inhibitory to yeast metabolism. This study compares the fermentation behaviours of two yeast strains using different types of lignocellulosic substrates; high gravity dilute acid spruce hydrolysate (SH) and spent sulphite liquor (SSL), in the absence and presence of yeast extract. To this end, the fermentation performance, energy status and fermentation capacity of the strains were measured under different growth conditions.

Results

Nutrient supplementation with yeast extract increased sugar uptake, cell growth and ethanol production in all tested fermentation conditions, but had little or no effect on the energy status, irrespective of media. Nutrient-supplemented medium enhanced the fermentation capacity of harvested cells, indicating that cell viability and reusability was increased by nutrient addition.

Conclusions

Although both substrates belong to the lignocellulosic spruce hydrolysates, their differences offer specific challenges and the overall yields and productivities largely depend on choice of fermenting strain.

【 授权许可】

   
2014 Johansson et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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【 参考文献 】

• [1]Jeon YJ, Xun Z, Rogers PL: Comparative evaluations of cellulosic raw materials for second generation bioethanol production. Lett Appl Microbiol 2010, 51:518-524.
• [2]Sjöström E: Wood chemistry fundamentals and applications. Orlando: Academic Press, INC; 1981.
• [3]Sluiter JB, Ruiz RO, Scarlata CJ, Sluiter AD, Templeton DW: Compositional analysis of lignocellulosic feedstocks. 1. Review and description of methods. J Agric Food Chem 2010, 58:9043-9053.
• [4]Palmqvist E, Hahn-Hägerdal B: Fermentation of lignocellulosic hydrolysates II: inhibitors and mechanisms of inhibition. Bioresour Technol 2000, 74:25-33.
• [5]Almeida JRM, Bertilsson M, Gorwa-Grauslund MF, Gorsich S, Lidén G: Metabolic effects of furaldehydes and impacts on biotechnological processes. Appl Microbiol Biotechnol 2009, 82:625-638.
• [6]Larsson S, Palmqvist E, Hahn-Hägerdal B, Tengborg C, Stenberg K, Zacchi G, Nilvebrant N-O: The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb Technol 1999, 24:151-159.
• [7]Verduyn C, Postma E, Scheffers WA, Dijken JP: Effect of benzoic acid on metabolic fuxes in yeasts: a continous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 1992, 8:501-517.
• [8]Jones RP: Biological principles for the effects of ethanol. Enzyme Microb Technol 1989, 11:130-153.
• [9]Heipieper HJ, Weber FJ, Sikkema J, Kewelo H, de Bont JAM: Mechanism of resistance of whole cells to toxic organic solvents. Trends Biotech 1994, 12:409-415.
• [10]Johansson E, Brandberg T, Larsson C: Influence of cultivation procedure for Saccharomyces cerevisiae used as pitching agent in industrial spent sulphite liquid fermentations. J Ind Microbiol Biotechnol 2011, 38:1787-1792.
• [11]Jones AM, Ingledew WM: Fuel alcohol production: appraisal of nitrogenous yeast foods for very high gravity wheat mash fermentation. Process Biochem 1994, 29:483-488.
• [12]Casey GP, Magnus CA, Ingledew WM: High-gravity brewing: effects of nutrition on yeast composition, fermentative ability, and alcohol production. Appl Environ Microbiol 1984, 48:639-646.
• [13]Thomas KC, Ingledew WM: Fuel alcohol production: effects of free amino nitrogen on fermentation of very-high-gravity wheat mashes. Appl Environ Microbiol 1990, 56:2046-2050.
• [14]Albers E, Larsson C, Lidén G, Niklasson C, Gustafsson L: Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation. Appl Environ Microbiol 1996, 62:3187-3195.
• [15]Devantier R, Scheithauer B, Granato Villas-Boâs S, Pedersen S, Olsson L: Metabolite profiling for analysis of yeast stress response during very high gravity ethanol fermentations. Biotechnol Bioeng 2005, 90:703-714.
• [16]Bayrock DP, Ingledew WM: Application of multistage continuous fermentation for production of fuel alcohol by very-high-gravity fermentation technology. J Ind Microbiol Biotechnol 2001, 27:87-93.
• [17]Galbe M, Sassner P, Wingren A, Zacchi G: Process engineering economics of bioethanol production. In Adv Biochem Eng Biotechnol. Volume 108. Edited by Olsson L. Heidelberg: Springer-Verlag; 2007:303-327.
• [18]Lau MW, Dale BE, Balan V: Ethanolic fermentation of hydrolysates from ammonia fiber expansion (AFEX) treated corn stover and distillers grain without detoxification and external nutrient supplementation. Biotechnol Bioeng 2008, 99:529-539.
• [19]Alkasrawi M, Rudolf A, Liden G, Zacchi G: Influence of strain and cultivation procedure on the performance of simultaneous saccharification and fermentation of steam pretreated spruce. Enzyme Microb Technol 2006, 38:279-286.
• [20]Gasch AP: The Environmental Stress Response: A Common Yeast Response to Environmental Stresses. In Yeast Stress Responses. Volume 1. Edited by Hohmann S, Mager P. Heidelberg: Springer-Verlag; 2002:11-70.
• [21]Sárvári Horváth I, Franzén CJ, Taherzadeh MJ, Niklasson C, Lidén G: Effects of furfural on the respiratory metabolism of Saccharomyces cerevisiae in glucose-limited chemostats. Appl Environ Microbiol 2003, 69:4076-4086.
• [22]Nielsen J: It is all about metabolic fluxes. J Bacteriol 2003, 185:7031-7035.
• [23]Lallemand product sheet Thermosacc Dry [http://www.lallemandbds.com/wp-content/uploads/2012/12/2013_LBDS_Data-Sheet_Thermosacc-Dry_10080_Data_Rev.00.01.01.2013.pdf webcite]
• [24]Purwadi R, Brandberg T, Taherzadeh : A possible industrial solution to ferment lignocellulosic hydrolysates to ethanol: Continuous cultivation with flocculating yeast. Int J Mol Sci 2007, 8:920-932.
• [25]Canelas AB, Ras C, Pierick A, Dam JC, Heijnen JJ, Gulik WM: Leakage-free rapid quenching technique for yeast metabolomics. Metabolomics 2008, 4:226-239.
• [26]Lundin A, Thore A: Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay. Appl Microbiol 1975, 30:713-721.
• [27]Atkinson DE: The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochem 1968, 7:4030-4034.
• [28]Olofsson K, Bertilsson M, Liden G: A short review on SSF - an interesting process option for ethanol production from lignocellulosic feedstocks. Biotechnol Biofuels 2008, 1:7. BioMed Central Full Text
• [29]Meneses FJ, Henschke PA, Jiranek V: A survey of industrial strains of Saccharomyces cerevisiae reveals numerous altered patterns of maltose and sucrose utilisation. J Inst Brew 2002, 108:310-321.
• [30]Martin C, Jönsson LJ: Comparison of the resistance of industrial and laboratory strains of Saccharomyces and Zygosaccharomyces to lignocellulose-derived fermentation inhibitors. Enzyme Microb Technol 2003, 32:386-395.
• [31]Modig T, Almeida JRM, Gorwa-Grauslund MF, Lidén G: Variability of the response of Saccharomyces cerevisiae strains to lignocellulose hydrolysate. Biotechnol Bioeng 2008, 100:423-429.
• [32]Stanley D, Bandara A, Fraser S, Chambers PJ, Stanley GA: The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae. J Appl Microbiol 2010, 109:13-24.
• [33]Foster PL: Adaptive mutation - the uses of adversity. Annu Rev Microbiol 1993, 47:467-504.
• [34]Rosenberg SM: Evolving responsively: adaptive mutation. Nat Rev Genet 2001, 2:645-645.
• [35]Blomberg A, Adler L: Physiology of osmotolerance in fungi. Adv Microb Physiol 1992, 33:145-212.
• [36]Erasmus DJ, van der Merwe GK, van Vuuren HJJ: Genome-wide expression analyses: metabolic adaptation of Saccharomyces cerevisiae to high sugar stress. FEMS Yeast Res 2003, 3:375-399.
• [37]Gray WD: Studies on the alcohol tolerance of yeasts. J Bacteriol 1941, 42:561-574.
• [38]Alfenore S, Molina-Jouve C, Guillouet SE, Uribelarrea J-L, Goma G, Benbadis L: Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process. Appl Microbiol Biotechnol 2002, 60:67-72.
• [39]Jorgensen H: Effect of nutrients on fermentation of pretreated wheat straw at very high dry matter content. Appl Biochem Biotechnol 2009, 153:44-57.
• [40]Gustafsson L, Ölz R, Larsson K, Larsson C, Adler L: Energy balancing calculations as a tool to determine maintenance energy requirements under stress conditions. J Pure Appl Chem 1993, 65:1893-1898.
• [41]Larsson C, Nilsson A, Blomberg A, Gustafsson L: Glycolytic flux is conditionally correlated with ATP concentration in Saccharomyces cerevisiae: a chemostat study under carbon- or nitrogen-limiting conditions. J Bacteriol 1997, 179:7243-7250.
• [42]Larsson C, Påhlman I-L, Gustafsson L: The importance of ATP as a regulator of glycolytic flux in Saccharomyces cerevisiae. Yeast 2000, 16:797-809.