Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw

Biotechnology for Biofuels

Yubin Zheng¹  Xiaochen Yu¹  Jijiao Zeng¹  Shulin Chen¹ 

[1] Department of Biological Systems Engineering, L.J. Smith Hall, Washington State University, Pullman, WA, 99164-6120, USA

关键词: Wheat straw;    Lignocellulosic biomass;    Biodiesel;    Microbial lipid;    Mortierella isabellina;    Filamentous fungi;   

Others  :  798261
DOI  :  10.1186/1754-6834-5-50

 received in 2012-04-26, accepted in 2012-07-02,  发布年份 2012

【 摘 要 】

Background

Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstock for biofuel production due to its abundance and low value. However, very limited study has been performed on lipid production by culturing oleaginous fungi with lignocellulosic materials. Thus, identification of filamentous fungal strains capable of utilizing lignocellulosic hydrolysates for lipid accumulation is critical to improve the process and reduce the production cost.

Results

The growth performances of eleven filamentous fungi were investigated when cultured on glucose and xylose. Their dry cell weights, lipid contents and fatty acid profiles were determined. Six fungal strains with high lipid contents were selected to culture with the hydrolysate from dilute sulfuric acid pretreatment of wheat straw. The results showed that all the selected fungal strains were able to grow on both detoxified liquid hydrolysate (DLH) and non-detoxified liquid hydrolysate (NDLH). The highest lipid content of 39.4% was obtained by Mortierella isabellina on NDLH. In addition, NDLH with some precipitate could help M. isabellina form pellets with an average diameter of 0.11 mm.

Conclusion

This study demonstrated the possibility of fungal lipid production from lignocellulosic biomass. M. isabellina was the best lipid producer grown on lignocellulosic hydrolysates among the tested filamentous fungi, because it could not only accumulate oils with a high content by directly utilizing NDLH to simplify the fermentation process, but also form proper pellets to benefit the downstream harvesting. Considering the yield and cost, fungal lipids from lignocellulosic biomass are promising alternative sources for biodiesel production.

【 授权许可】

   
2012 Zheng et al.; licensee BioMed Central Ltd.

【 图 表 】

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