Photo-hydrogen and lipid production from lactate, acetate, butyrate, and sugar manufacturing wastewater with an alternative nitrogen source by Rhodobacter sp . KKU-PS1

PeerJ

article

Thitirut Assawamongkholsiri¹  Alissara Reungsang²  Sureewan Sittijunda⁴ 

[1] Research and Development of GM Plant & Microbe Detection Laboratory/Biotechnology Research and Development Office, Department of Agriculture;Department of Biotechnology/Faculty of Technology/Khon Kaen University;Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University;Faculty of Environment and Resource Studies, Mahidol University

关键词: Bio-hydrogen;    Microbial lipid;    Photo fermentation;    Volatile fatty acids;    Purple non-sulfur photosynthetic bacteria;   

DOI  :  10.7717/peerj.6653

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Photo-hydrogen and lipid production from individual synthetic volatile fatty acids (VFAs) and sugar manufacturing wastewater (SMW) by Rhodobacter sp. KKU-PS1 with sodium glutamate or Aji-L (i.e., waste from the process of crystallizing monosodium glutamate) as a nitrogen source was investigated. Using individual synthetic VFAs, the maximum hydrogen production was achieved with Aji-L as a nitrogen source rather than sodium glutamate. The maximum hydrogen production was 1,727, 754 and 1,353 mL H2/L, respectively, using 25 mM of lactate, 40 mM of acetate and 15mM of butyrate as substrates. Under these conditions, lipid was produced in the range of 10.6–16.9% (w/w). Subsequently, photo-hydrogen and lipid production from SMW using Aji-L as nitrogen source was conducted. Maximal hydrogen production and hydrogen yields of 1,672 mL H2/L and 1.92 mol H2/mol substrate, respectively, were obtained. Additionally, lipid content and lipid production of 21.3% (w/w) and 475 mg lipid/L were achieved. The analysis of the lipid and fatty acid components revealed that triacyglycerol (TAG) and C18:1 methyl ester were the main lipid and fatty acid components, respectively, found in Rhodobacter sp. KKU-PS1 cells.

【 授权许可】

CC BY