Prototype of biliary drug-eluting stent with photodynamic and chemotherapy using electrospinning

BioMedical Engineering OnLine

Min-Hua Chen³  Po-Chin Liang³  Kai-Chun Chang⁵  Jian-Yuan Huang³  Yu-Ting Chang¹  Fuh-Yu Chang⁴  Jau-Min Wong³  Feng-Huei Lin² 

[1] Department of Internal Medicine, National Taiwan University Hospital, Taipei 10051, Taiwan

[2] Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County 35053, Taiwan

[3] Institute of Biomedical Engineering, National Taiwan University, No.1, Sec.1, Jen-Ai Rd., Taipei 100, Taiwan

[4] Department of Mechanical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan

[5] Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei 10051, Taiwan

关键词: Electrospinning and Electrospraying;    Biliary drug-eluting stent;    Chemotherapy;    Photodynamic therapy;    Cholangiocarcinoma;   

Others  :  1084515
DOI  :  10.1186/1475-925X-13-118

 received in 2014-04-15, accepted in 2014-08-14,  发布年份 2014

【 摘 要 】

Background

The combination of biliary stent with photodynamic and chemotherapy seemed to be a beneficial palliative treatment of unresectable cholangiocarcinoma. However, by intravenous delivery to the target tumor the distribution of the drug had its limitations and caused serious side effect on non-target organs. Therefore, in this study, we are going to develop a localized eluting stent, named PDT-chemo stent, covered with gemcitabine (GEM) and hematoporphyrin (HP).

Methods

The prototype of PDT-chemo stent was made through electrospinning and electrospraying dual-processes with an electrical charge to cover the stent with a drug-storing membrane from polymer liquid. The design of prototype used PU as the material of the backing layer, and PCL/PEG blends in different molar ratio of 9:1 and of 1:4 were used in two drug-storing layers with GEM and HP loaded respectively.

Results

The optical microscopy revealed that the backing layer was formed in fine fibers from electrospinning, while drug-storing layers, attributed to the droplets from electrospraying process. The covered membrane, the morphology of which was observed by scanning electron microscopy (SEM), covered the stent surface homogeneously without crack appearances. The GEM had almost 100% of electrosprayed efficiency than 70% HP loaded on the covered membrane due to the different solubility of drug in PEG/PCL blends. Drug release study confirmed the two-phased drug release pattern by regulating in different molar ratio of PEG/PCL blends polymer.

Conclusions

The result proves that the PDT-chemo stent is composed of a first burst-releasing phase from HP and a later slow-releasing phase from GEM eluting. This two-phase of drug eluting stent may provide a new prospect of localized and controlled release treatment for cholangiocarcinoma disease.

【 授权许可】

   
2014 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

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