Solution casting of chitosan membranes for in vitro evaluation of bioactivity

Biological Procedures Online

Ramona Lieder¹  Mariam Darai¹  Gissur Örlygsson¹  Olafur E Sigurjonsson² 

[1] Innovation Center Iceland, Arleynir 2-8, 112 Reykjavik, Iceland

[2] Biomedical Center, University of Iceland, Vatnsmyrarvegur 16, 101 Reykjavik, Iceland

关键词: Crosslinking;    Fibronectin adsorption;    MC3T3-E1;    Chitosan derivatives;    Titanium;    Characterization;    Membranes;    Chitosan;   

Others  :  793028
DOI  :  10.1186/1480-9222-15-11

 received in 2013-10-01, accepted in 2013-10-05,  发布年份 2013

【 摘 要 】

Background

Considerable research is focusing on the surface modification of titanium implants for the treatment of orthopaedic tissue injuries to increase the success of orthopaedic fixations. Chitosan is one of the natural materials under investigation based on several favourable properties. Numerous techniques have been described for the preparation of chitosan membranes, including solution casting methods for the investigation of bioactivity before applying coatings onto potential titanium implants. Solution casting enables the easy in-house evaluation of chitosan membranes and allows for the selection of promising chitosan materials.

Results

We present a method for the standardized and easily applied preparation of chitosan membranes by solution casting. This protocol is suitable for chitosan materials spanning a wide degree of deacetylation, being derived from different chitin sources and chitosan derivatives with novel properties. We detail the preparation and quality control methods in order to prepare membranes with favourable bioactivity, sustaining cell attachment and proliferation for extended culture periods.

Conclusions

The possibilities associated with the use of chitosan in tissue engineering applications are far from being exhausted and numerous challenges remain prior to successful translation into the clinics. Based on our experience, we have developed simple in-house methods for quality control of homogeneous membrane casting and early prediction of successful experimental outcome.

【 授权许可】

   
2013 Lieder et al.; licensee BioMed Central Ltd.

【 图 表 】

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