Bio-butanol production from glycerol with Clostridium pasteurianum CH4: the effects of butyrate addition and in situ butanol removal via membrane distillation

Biotechnology for Biofuels

De-Shun Lin²  Hong-Wei Yen⁴  Wei-Chen Kao²  Chieh-Lun Cheng²  Wen-Ming Chen¹  Chieh-Chen Huang⁵  Jo-Shu Chang³ 

[1] Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan

[2] Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan

[3] Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan

[4] Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan

[5] Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

关键词: Glycerol;    Butyrate addition;    Vacuum membrane distillation (VMD);    Clostridium pasteurianum;    Bio-butanol;   

Others  :  1229684
DOI  :  10.1186/s13068-015-0352-6

 received in 2015-04-21, accepted in 2015-09-30,  发布年份 2015

【 摘 要 】

Background

Clostridium pasteurianum CH4 was used to produce butanol from glycerol. The performance of butanol fermentation was improved by adding butyrate as the precursor to trigger the metabolic pathway toward butanol production, and by combining this with in situ butanol removal via vacuum membrane distillation (VMD) to avoid the product inhibition arising from a high butanol concentration.

Results

Adding 6 g L ⁻¹butyrate as precursor led to an increase in the butanol yield from 0.24 to 0.34 mol butanol (mol glycerol) ⁻¹ . Combining VMD and butyrate addition strategies could further enhance the maximum effective butanol concentration to 29.8 g L ⁻¹ , while the yield was also improved to 0.39 mol butanol (mol glycerol) ⁻¹ . The butanol concentration in the permeate of VMD was nearly five times higher than that in the feeding solution.

Conclusions

The proposed butyrate addition and VMD in situ butanol removal strategies are very effective in enhancing both butanol titer and butanol yield. This would significantly enhance the economic feasibility of fermentative production of butanol. The VMD-based technology not only alleviates the inhibitory effect of butanol, but also markedly increases butanol concentration in the permeate after condensation, thereby making downstream processing easier and more cost-effective.

【 授权许可】

   
2015 Lin et al.

【 图 表 】

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