Phylogeny-structured carbohydrate metabolism across microbiomes collected from different units in wastewater treatment process

Biotechnology for Biofuels

Tong Zhang¹  Yuanqing Chao³  Francis Y. L. Chin²  Yu Xia¹ 

[1]Environmental Biotechnology Laboratory, The University of Hong Kong, Hong Kong, SAR, China

[2]Department of Computing, Hang Seng Management College, Hong Kong, SAR, China

[3]School of Environmental Science and Engineering, Sun Yat-sen University, Guangdong, China

关键词: Salinity;    Dissolved oxygen;    Temperature;    Glycoside hydrolase;    Carbohydrate metabolism;    Metagenomic;   

Others  :  1229676
DOI  :  10.1186/s13068-015-0348-2

 received in 2015-08-06, accepted in 2015-09-25,  发布年份 2015

【 摘 要 】

Background

With respect to global priority for bioenergy production from plant biomass, understanding the fundamental genetic associations underlying carbohydrate metabolisms is crucial for the development of effective biorefinery process. Compared with gut microbiome of ruminal animals and wood-feed insects, knowledge on carbohydrate metabolisms of engineered biosystems is limited.

Results

In this study, comparative metagenomics coupled with metabolic network analysis was carried out to study the inter-species cooperation and competition among carbohydrate-active microbes in typical units of wastewater treatment process including activated sludge and anaerobic digestion. For the first time, sludge metagenomes demonstrated rather diverse pool of carbohydrate-active genes (CAGs) comparable to that of rumen microbiota. Overall, the CAG composition correlated strongly with the microbial phylogenetic structure across sludge types. Gene-centric clustering analysis showed the carbohydrate pathways of sludge systems were shaped by different environmental factors, including dissolved oxygen and salinity, and the latter showed more determinative influence of phylogenetic composition. Eventually, the highly clustered co-occurrence network of CAGs and saccharolytic phenotypes, revealed three metabolic modules in which the prevalent populations of Actinomycetales, Clostridiales and Thermotogales, respectively, play significant roles as interaction hubs, while broad negative co-exclusion correlations observed between anaerobic and aerobic microbes, probably implicated roles of niche separation by dissolved oxygen in determining the microbial assembly.

Conclusions

Sludge microbiomes encoding diverse pool of CAGs was another potential source for effective lignocellulosic biomass breakdown. But unlike gut microbiomes in which Clostridiales, Lactobacillales and Bacteroidales play a vital role, the carbohydrate metabolism of sludge systems is built on the inter-species cooperation and competition among Actinomycetales, Clostridiales and Thermotogales.

【 授权许可】

   
2015 Xia et al.

【 图 表 】

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