Mechanistic study of a diazo dye degradation by Soybean Peroxidase

Chemistry Central Journal

Umme Kalsoom¹  Syed Salman Ashraf²  Mohammed A Meetani²  Muhammad A Rauf²  Haq Nawaz Bhatti¹ 

[1] Department of Chemistry & Biochemistry, University of Agriculture, Faisalabad, Pakistan

[2] Department of Chemistry, UAE University, P. O. Box 15551, Al-Ain, United Arab Emirates

关键词: LC-MS/MS;    Soybean peroxidase;    Mechanism;    Degradation;    Enzymes;    Phenol dye;    Trypan Blue;    Azo dye;   

Others  :  787900
DOI  :  10.1186/1752-153X-7-93

 received in 2013-03-28, accepted in 2013-05-16,  发布年份 2013

【 摘 要 】

Background

Enzyme based remediation of wastewater is emerging as a novel, efficient and environmentally-friendlier approach. However, studies showing detailed mechanisms of enzyme mediated degradation of organic pollutants are not widely published.

Results

The present report describes a detailed study on the use of Soybean Peroxidase to efficiently degrade Trypan Blue, a diazo dye. In addition to examining various parameters that can affect the dye degradation ability of the enzyme, such as enzyme and H₂O₂ concentration, reaction pH and temperature, we carried out a detailed mechanistic study of Trypan Blue degradation. HPLC-DAD and LC-MS/MS studies were carried out to confirm dye degradation and analyze the intermediate metabolites and develop a detailed mechanistic dye degradation pathway.

Conclusion

We report that Soybean peroxidase causes Trypan Blue degradation via symmetrical azo bond cleavage and subsequent radical-initiated ring opening of the metabolites. Interestingly, our results also show that no high molecular weight polymers were produced during the peroxidase-H₂O₂ mediated degradation of the phenolic Trypan Blue.

【 授权许可】

   
2013 Kalsoom et al.; licensee Chemistry Central Ltd.

【 图 表 】

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