Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes

Biotechnology for Biofuels

Anthony Levasseur²  Elodie Drula¹  Vincent Lombard¹  Pedro M Coutinho¹  Bernard Henrissat¹ 

[1] Architecture et Fonction des Macromolécules Biologiques, UMR6098, CNRS, Aix-Marseille Université, 163 Avenue de Luminy, Marseille, 13288, France

[2] INRA, UMR1163 Biotechnologie des Champignons Filamenteux, Aix-Marseille Université, ESIL Polytech Marseille, 163 avenue de Luminy, CP 925, 13288, Marseille, Cedex 09, France

关键词: Lytic polysaccharide monooxygenases;    Ligninolytic enzymes;    Evolution of lignocellulose breakdown;    CAZy database;   

Others  :  798116
DOI  :  10.1186/1754-6834-6-41

 received in 2013-01-24, accepted in 2013-03-11,  发布年份 2013

【 摘 要 】

Background

Since its inception, the carbohydrate-active enzymes database (CAZy; http://www.cazy.org webcite) has described the families of enzymes that cleave or build complex carbohydrates, namely the glycoside hydrolases (GH), the polysaccharide lyases (PL), the carbohydrate esterases (CE), the glycosyltransferases (GT) and their appended non-catalytic carbohydrate-binding modules (CBM). The recent discovery that members of families CBM33 and family GH61 are in fact lytic polysaccharide monooxygenases (LPMO), demands a reclassification of these families into a suitable category.

Results

Because lignin is invariably found together with polysaccharides in the plant cell wall and because lignin fragments are likely to act in concert with (LPMO), we have decided to join the families of lignin degradation enzymes to the LPMO families and launch a new CAZy class that we name “Auxiliary Activities” in order to accommodate a range of enzyme mechanisms and substrates related to lignocellulose conversion. Comparative analyses of these auxiliary activities in 41 fungal genomes reveal a pertinent division of several fungal groups and subgroups combining their phylogenetic origin and their nutritional mode (white vs. brown rot).

Conclusions

The new class introduced in the CAZy database extends the traditional CAZy families, and provides a better coverage of the full extent of the lignocellulose breakdown machinery.

【 授权许可】

   
2013 Levasseur et al.; licensee BioMed Central Ltd.

【 图 表 】

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