Staphylococcal enterotoxin B influences the DNA methylation pattern in nasal polyp tissue: a preliminary study

Allergy, Asthma & Clinical Immunology

Claudina A Pérez-Novo⁴  Yuan Zhang³  Simon Denil²  Geert Trooskens²  Tim De Meyer²  Wim Van Criekinge²  Paul Van Cauwenberge⁴  Luo Zhang¹  Claus Bachert⁵ 

[1] Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China

[2] Department of Mathematical Modelling, Statistics and Bioinformatics, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

[3] Key Laboratory of Otolaryngology, Head and Neck Surgery (Ministry of Education of China), Beijing Institute of Otorhinolaryngology, Beijing 100005, PR China

[4] Upper Airways Research Laboratory, Department of Otorhinolaryngology, Ghent University Hospital, De Pintelaan 185, Ghent B-9000, Belgium

[5] Karolinska Institutet, Division of ENT Diseases, CLINTEC, Stockholm, Sweden

关键词: Hypermethylation;    Whole genome methylation analysis;    MBD2;    DNA methylation;    Chronic rhinosinusitis and nasal polyps;    Staphylococcus aureus enterotoxin B;   

Others  :  792050
DOI  :  10.1186/1710-1492-9-48

 received in 2013-09-20, accepted in 2013-12-09,  发布年份 2013

【 摘 要 】

Staphylococcal enterotoxins may influence the pro-inflammatory pattern of chronic sinus diseases via epigenetic events. This work intended to investigate the potential of staphylococcal enterotoxin B (SEB) to induce changes in the DNA methylation pattern. Nasal polyp tissue explants were cultured in the presence and absence of SEB; genomic DNA was then isolated and used for whole genome methylation analysis. Results showed that SEB stimulation altered the methylation pattern of gene regions when compared with non stimulated tissue. Data enrichment analysis highlighted two genes: the IKBKB and STAT-5B, both playing a crucial role in T- cell maturation/activation and immune response.

【 授权许可】

   
2013 Pérez-Novo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Bachert C, Van SK, Zhang N, Holtappels G, Cattaert T, Maus B, Buhl R, Taube C, Korn S, Kowalski M: Specific IgE against Staphylococcus aureus enterotoxins: an independent risk factor for asthma. J Allergy Clin Immunol 2012, 130:376-381.
• [2]Fokkens WJ, Lund VJ, Mullol J, Bachert C, Alobid I, Baroody F, Cohen N, Cervin A, Douglas R, Gevaert P, et al.: European position paper on rhinosinusitis and nasal polyps. Rhinol Suppl 2012, 2012:3-298.
• [3]Bierne H, Hamon M, Cossart P: Epigenetics and bacterial infections. Cold Spring Harb Perspect Med 2012, 2:a010272.
• [4]Patou J, Gevaert P, Van ZT, Holtappels G, Van CP, Bachert C: Staphylococcus aureus enterotoxin B, protein A, and lipoteichoic acid stimulations in nasal polyps. J Allergy Clin Immunol 2008, 121:110-115.
• [5]Hardcastle TJ, Kelly KA: baySeq: empirical Bayesian methods for identifying differential expression in sequence count data. BMC Bioinforma 2010, 11:422. BioMed Central Full Text
• [6]Langmead B, Trapnell C, Pop M, Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 2009, 10:R25. BioMed Central Full Text
• [7]D'Eustachio P: Reactome knowledgebase of human biological pathways and processes. Methods Mol Biol 2011, 694:49-61.
• [8]Shiomori T, Yoshida S, Miyamoto H, Makishima K: Relationship of nasal carriage of Staphylococcus aureus to pathogenesis of perennial allergic rhinitis. J Allergy Clin Immunol 2000, 105:449-454.