Cryptosporidiosis – an occupational risk and a disregarded disease in Estonia

Acta Veterinaria Scandinavica

Brian Lassen¹  Marie Ståhl²  Heidi L Enemark² 

[1] Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51014 Tartu, Estonia

[2] Technical University of Denmark, DK-1870 Frederiksberg C, Denmark

关键词: Cattle;    Zoonoses;    Human;    Genotype;    Estonia;    Cryptosporidium parvum;   

Others  :  1082589
DOI  :  10.1186/1751-0147-56-36

 received in 2014-01-21, accepted in 2014-05-20,  发布年份 2014

【 摘 要 】

Background

Cases of cryptosporidiosis have not been officially reported in Estonia after the year 2000, and the disease appears to be either under-diagnosed or under-reported.

Findings

Based on a human case of cryptosporidiosis contracted during faecal sampling in dairy farms, cattle considered to be sources of infection were analysed for Cryptosporidium spp. by a modified Ziehl Neelsen technique and molecular typing. C. parvum subtype IIaA16G1R1 was detected from the human case and from calves from one of nine farms enrolled in the study providing strong circumstantial evidence of zoonotic transmission from calves to humans.

Conclusion

Cryptosporidiosis presents an occupational risk to people with cattle contact, and may also be a risk to the human population in general. Thus increased public and medical awareness is warranted.

【 授权许可】

   
2014 Lassen et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Chako CZ, Tyler JW, Schultz LG, Chiguma L, Beerntsen BT: Cryptosporidiosis in people: it’s not just about the cows. J Vet Intern Med 2010, 24:37-43.
• [2]Lassen B, Viltrop A, Raaperi K, Järvis T: Eimeria and Cryptosporidium in Estonian dairy farms in regard to age, species, and diarrhoea. Vet Parasitol 2009, 166:212-219.
• [3]Estonian. Veterinary Diagnostic and Food Laboratories (VAFL): Aastaaruaned. 2012. [http://www.vetlab.ee webcite]
• [4]European Centre for Disease Prevention and Control (ECDC): Annual Epidemiological Report 2011. Reporting on 2009 Surveillance Data and 2010 Epidemic Intelligence Data. Stockholm: ECDC; 2011:77-79.
• [5]Estonian Health Board: Nakkushaigused Eestis. [http://www.terviseamet.ee/nakkushaigused/nakkushaigustesse-haigestumine.html webcite]
• [6]Henriksen SA, Pohlenz JF: Staining of cryptosporidia by a modified Ziehl-Neelsen technique. Acta Vet Scand 1981, 22:594-596.
• [7]Enemark HL, Ahrens P, Juel CD, Petersen E, Petersen RF, Andersen JS, Lind P, Thamsborg SM: Molecular characterization of Danish Cryptosporidium parvum isolates. Parasitology 2002, 125:331-341.
• [8]Morgan UM, Monis PT, Xiao L, Limor J, Sulaiman I, Raidal S, O’Donoghue P, Gasser R, Murray A, Fayer R, Blaqburn BL, Lal AA, Thompson RC: Molecular and phylogenetic characterisation of Cryptosporidium from birds. Int J Parasitol 2001, 31:289-296.
• [9]Peng MM, Wilson ML, Holland RE, Meshnick SR, Lal AA, Xiao L: Genetic diversity of Cryptosporidium spp. in cattle in Michigan: implications for understanding the transmission dynamics. Parasitol Res 2003, 90:175-180.
• [10]Sulaiman IM, Hira PR, Zhou L, Al-Ali FM, Al-Shelahi FA, Shweiki HM, Iqbal J, Khalid N, Xiao L: Unique endemicity of cryptosporidiosis in children in Kuwait. J Clin Microbiol 2005, 43:2805-2809.
• [11]Imre K, Lobo LM, Matos O, Popescu C, Genchi C, Dărăbuş G: Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania: is there an actual risk of zoonotic infections? Vet Parasitol 2011, 181:321-324.
• [12]Kváč M, Hromadová N, Květoňová D, Rost M, Sak B: Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republich: absence of C. ryanae and management-associated distriburion of C. andersoni, C. bovis and C. parvum subtypes. Vet Parasitol 2011, 177:378-382.
• [13]Ondrácková Z, Kvác M, Sak B, Kvetonová D, Rost M: Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic. Vet Parasitol 2009, 165:141-144.
• [14]Soba B, Logar J: Genetic classification of Cryptosporidium isolates from humans and calves in Slovenia. Parasitology 2008, 135:1263-1270.
• [15]Plutzer J, Karanis P: Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary. Vet Parasitol 2007, 146:357-362.
• [16]Imre K, Luca C, Costache M, Sala C, Morar A, Morariu S, Ilie MS, Imre M, Dărăbuş G: Zoonotic Cryptosporidium parvum in Romanian newborn lambs (Ovis aries). Vet Parasitol 2013, 191:119-122.
• [17]Kvác M, Sak B, Hanzlíková D, Kotilová J, Kvetonová D: Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic. Parasitol Res 2009, 104:425-428.
• [18]Lassen B: Diagnosis, Epidemiology and Control of Bovine Coccidioses in Estonia. PhD Thesis. Tartu: Ecoprint; 2009.
• [19]Dixon B, Parrington L, Cook A, Pintar K, Pollari F, Kelton D, Farber J: The potential for zoonotic transmission of Giardia duodenalis and Cryptosporidium spp. from beef and dairy cattle in Ontario, Canada. Vet Parasitol 2011, 175:20-26.
• [20]Langkjaer RB, Vigre H, Enemark HL, Maddox-Hyttel C: Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark. Parasitology 2007, 134:339-350.
• [21]Gait R, Soutar RH, Hanson M, Fraser C, Chalmers R: Outbreak of cryptosporidiosis among veterinary students. Vet Rec 2008, 162:843-845.
• [22]Autio T, Karhukorpi J, Mäkelä M, Meri T, Savolainen S, Rimhanen-Finne R: Endemic cryptosporidiosis–underdiagnosed disease in Finland. Duodecim 2012, 28:1887-1890.
• [23]Gatei W, Suputtamongkol Y, Waywa D, Ashford RW, Bailey JW, Greensill J, Beeching NJ, Hart CA: Zoonotic species of Cryptosporidium are as prevalent as the anthroponotic in HIV-infected patients in Thailand. Ann Trop Med Parasitol 2002, 96:797-802.
• [24]Izadi M, Jonaidi-Jafari N, Saburi A, Eyni H, Rezaiemanesh MR, Ranjbar R: Prevalence, molecular characteristics and risk factors for cryptosporidiosis among Iranian immunocompromised patients. Microbiol Immunol 2012, 56:836-842.
• [25]Laisaar KT, Avi R, DeHovitz J, Uusküla A: Estonia at the threshold of the fourth decade of the AIDS era in Europe. AIDS Res Hum Retroviruses 2011, 27:841-851.