Influence of AC electric field on the charge generation in albumin solution in a flow-based AFM fishing system

Scanning Probe Microscopy 2018

Ivanov, Y.D.^1 ; Kozlov, A.F.^1 ; Galiullin, R.A.^1 ; Tatur, V.Y.^2 ; Ivanova, N.D.^3 ; Ziborov, V.S.^4

Institute of Biomedical Chemistry, Moscow, Russia^1

Foundation of Perspective Technologies and Novations, Moscow, Russia^2

Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia^3

Joint Institute for High Temperatures RAS, Moscow, Russia^4

关键词: AC electric field;    Charge accumulation;    Charge generation;    Diagnostic systems;    Flow-based systems;    Physicochemical property;    Protein solution;    Sinusoidal electric fields;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/443/1/012011/pdf
DOI  :  10.1088/1757-899X/443/1/012011

来源: IOP

【 摘 要 】

In the present paper, the influence of AC electric field on charge accumulation in the measuring cell of a flow-based system for atomic force microscope (AFM) based fishing is considered. As a flow-based system, a device for AFM-based fishing of low-abundant proteins has been employed. The charge is generated in a femtomolar albumin solution during its flowing through an injector of this system. We have demonstrated that application of sinusoidal electric field (100 V, 50 Hz frequency) stimulates charge accumulation in the measuring cell upon the input of protein solution at 38?C. Taking this effect into account is of importance for the development of novel highly sensitive flow-based proteomic and diagnostic systems employing AFM-based fishing, for modeling the influence of electric field on hemodynamics, and also for the development of specified theoretical models describing physicochemical properties of water and aqueous solutions.