Identification of bacteria on Cockroach feet from Hospital area in Palu city and test of sensitivity to antibiotic

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Astiti, P.M.A.^1 ; Bialangi, M.^1 ; Kundera, I.N.^1

Departement of Biology Education, Faculty of Teacher Training and Education, Tadulako University Palu, Indonesia^1

关键词: Bacteria-resistant;    Bacterial identifications;    Bacterial isolation;    Biochemical tests;    Periplaneta americana;    Sensitivity tests;    Sensitivity to antibiotics;    Standard procedures;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012123/pdf
DOI  :  10.1088/1757-899X/434/1/012123

来源: IOP

【 摘 要 】

One of the mechanical vectors that co-exist with humans is the Cockroach (Periplaneta americana). Cockroaches act as a vector because they are closely related to the presence of bacteria carried on their feet. Cockroaches usually live mainly in homes, restaurants, hotels, hospitals, warehouses, offices, libraries, and elsewhere. These animals are thought to transmit various diseases and act as a vector or as a hospes. This research aims to identify the bacteria in Cockroach feet from Hospital area and test sensitivity to antibiotics. This research is laboratory exploratory, using the standard methods of bacterial isolation and identification, Gram staining, Microbact system KIT and antibiotic sensitivity test using "The Bauer-Kirby standard procedure". Data obtained from the isolation, Gram staining, biochemical test, and analyzed with microbact system 2000 software. The results of bacterial identification on Cockroach legs, which were obtained from three hospitals area in Palu, Klebsiella ozaenae, Salmoella arizona, Salmonella sp., Escherichia coli, Salmonella choleraesuis and Salmonella simultans. The results of sensitivity test to six of these bacteria against five kinds of antibiotics found all of these bacteria resistant to Vancomycin, Chloramphenicol, and Amoxicillin, but still sensitive to Ciprofloxacin and Ofloxacin.