Depolymerization and Assimilation of Poly (Ethylene Terephthalate) By Whole-Cell Bioprocess

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Gong, Jixian^1,2 ; Li, Yuqiang^1,2 ; Wang, Hongyang^1,2 ; Li, Huiqin^1,2 ; Zhang, Jianfei^1,2

School of Textiles, Tianjin Polytechnic University, Tianjin

300160, China^1

Key Laboratory of Advanced Textile Composites, Ministry of Education of China, Tianjin

300160, China^2

关键词: Amorphous regions;    Biofunctionalization;    Insoluble substrates;    Intermediate product;    Polyethylene terephthalates (PET);    Sole carbon source;    Synthetic polymers;    Whole cell biocatalysts;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/2/022047/pdf
DOI  :  10.1088/1757-899X/394/2/022047

来源: IOP

【 摘 要 】

Now, biofunctionalization of synthetic polymers can be achieved through biocatalysts. Uniquely among the great enzyme, whole-cell biocatalysts provide a promising alternative for bioprocess. The bioprocess of depolymerization and assimilation of polyethylene terephthalate (PET) was investigated with whole-cell from aeromonas as biocatalyst in this investigation. The engineered aeromonas strain, which can grow with PET particle as sole carbon source and major energy, was employed as biocatalysts. The main products, such as terephtalic acid (TA), bis (2-hydroxyethyl terephthalate) (BHET) and benzoic acid (BA), muconic acid (MA), were recognized in whole-cell bioprocess of PET depolymerization. It showed that variation of these products was more complex than that in case of enzyme treatment, for the interaction of solid insoluble substrate, microbe cell and enzymes secreted by cells in this heterogeneous process. The result demonstrated that not only the ester bonds can be cleaved, but also the benzene ring can be decomposed in the process of whole-cell catalysis. And some of the intermediate products, which have inhibiting effect in enzyme catalysis process, can be utilized and further degraded in whole-cell bioprocess. Furthermore, assays by DSC showed that as degradation of PET progressed, crystallinity of PET granular increased by 1.81% due to preferential degradation of amorphous regions.