Theoretical and Experimental Approach Towards P-Cyano Stilbene Schiff Base as a Potential Linker in E-DNA Sensor

Euroinvent ICIR 2018

Yusoff, H.M.^1,2 ; Hassan, N.^1,2 ; Ku Bulat, K.H.^2

Advanced Nanomaterials Research Group, School of Fundamental Science, Universiti Malaysia Terengganu, Terengganu, Kuala Terengganu

21030, Malaysia^1

School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Terengganu

21030, Malaysia^2

关键词: Electrochemical DNA sensor;    Experimental approaches;    Fourier transform infrared spectrometer;    Geometry optimization;    Schiff base compounds;    Schiff base molecules;    Stretching vibrations;    UV-Vis spectrophotometers;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/374/1/012086/pdf
DOI  :  10.1088/1757-899X/374/1/012086

来源: IOP

【 摘 要 】

This research aim to synthesis and characterize several p-cyano stilbene Schiff base molecules as a potential linker in electrochemical DNA sensor. Schiff base reaction is form from condensation reaction between primary amine and aldehyde at 79 °C using ethanol as solvent. All compounds are investigated and discussed by Fourier transform-infrared spectrometer (FTIR) and Uv-vis spectrophotometer. For computational study, structure drawings were constructed using GaussView 5.0. Geometry optimization has been carried out using density functional theory (DFT) at bB3LYP/6 31G and performed using Gaussian09 software. FTIR showed formation of C≡N stretching vibrations at 2224 cm-1also another important stretching at 1604 cm-1and 1249 cm-1which represent C=N and C-O mode respectively. In UV-vis, absorbance of C=N (imine) group can be observed at peak range 363 nm and transition of aromatic C=C can be seen at 238 nm. Theoretical and experimental results obtained are comparable and p-cyano stilbene Schiff base compounds were successfully synthesised and can be further applied as a linker in E-DNA sensor.