Biodegradation Capability of Some Bacteria Isolates to Use Lubricant Oil in Vitro

2nd International Conference on Mathematics, Science, Education and Technology

数学;自然科学;教育

Ahda, Y.^1 ; Azhar, M.^2 ; Fitri, L.^1 ; Afnida, A.^1 ; Adha, G.S.^1 ; Alifa, W.N.^1 ; Handayani, D.^1 ; Putri, D.H.^1 ; Irdawati, I.^1 ; Chatri, M.^1

Biology Department, Faculty of Mathematics and Sciences, Universitas Negeri Padang, Indonesia^1

Chemistry Department, Faculty of Mathematics and Sciences, Universitas Negeri Padang, Indonesia^2

关键词: Alcaligenes sp;    Degradation index;    In-situ characterization;    In-vitro;    Lubricant oil;    Neutral Red;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/335/1/012134/pdf
DOI  :  10.1088/1757-899X/335/1/012134

来源: IOP

【 摘 要 】

Our previous study identified three species of bacteria, i.e. Alcaligenes sp., Bacillus spl, and Bacillus sp2 isolated from using lubricant oil-contaminated soil in a Padang's workshop. However, its ability to degrade hydrocarbon were not known yet. In this extension study, we explore a wider area to find more hydrocarbonoclastic bacteria and examined its capability to degrade hydrocarbon in vitro. Seventeen isolates were characterized its capability using NA + used lubricant oil + tween + neutral red medium. Isolates A1, B2, D1 and D4 shows the high degradation index, whereas isolates A2, A3, A5, D2, B1, B3 and isolates A4, B4, D3 have medium and low degradation index, respectively. These potential hydrocarbonoclastic bacteria need in situ characterization to know their actual activities for bioremediation.