Isolation and identification of burn wound superbugs by molecular technique and their susceptibility to silver nanoparticles

International Conference on Advances in Materials and Manufacturing Applications 2017

Mala, R.^1 ; Ruby Celsia, A.S.^1

Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, Tamil Nadu

626005, India^1

关键词: Aqueous extracts;    Beta-lactum antibiotics;    Crystalline nature;    Diffusion method;    Global problems;    Inhibition zones;    Isolation and identification;    Molecular techniques;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/310/1/012146/pdf
DOI  :  10.1088/1757-899X/310/1/012146

来源: IOP

【 摘 要 】

Burn wound is a global problem affecting millions of people. It is the major cause of mortality and morbidity. This study was aimed to isolate and identify the wound isolates by 16S rRNA and to assess their susceptibility to antibiotics and silver nanoparticles. Silver nanoparticles were synthesized using aqueous extract of A.indica. The silver nanoparticles were characterized by FESEM, XRD, FTIR and DSC. Antibacterial susceptibility of the isolates was assessed by well diffusion method. The wound isolates were identified as S.aureus and E.coli. Both isolates were resistant to β lactum antibiotics, aminoglycoside, quinolones and macrolides. The inhibition zone exhibited by all antibiotics against both organisms was less than 5 mm. The size of silver nanoparticles were recorded as 55 nm. XRD confirmed the crystalline nature of the nanoparticles. TGA and DSC of silver nanoparticles showed the loss of weight and the melting point of silver nanoparticles was recorded at 871.3°C. Silver nano particles inhibited S.aureus and E.coli with an inhibition zone of 27 mm and 32 mm respectively. Therefore the study demonstrated that only silver containing dressings can be used in burn wounds infected by multi drug resistant super bugs.