Study of Horseradish Peroxidase Fixed on Mesoporous Materials as a Chemical Reaction Catalyst

1st International Conference on Frontiers of Materials Synthesis and Processing

材料科学;化学

Gao, Mengdan^1 ; Dai, Rongji^1

School of Beijing Institute of Technology, Beijing

100081, China^1

关键词: 1 ,3 ,5-Trimethylbenzene;    Enzymatic activities;    Horse-radish peroxidase;    Nano-structured;    Nitration reactions;    Oxidation reactions;    Polyoxypropylene;    Tetramethoxysilane;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/274/1/012058/pdf
DOI  :  10.1088/1757-899X/274/1/012058

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Nanostructured mesoporous materials is a new type of porous materials, which has been widely used. It has excellent capability in enzymes immobilization, but modification on the chemical bonds of the enzyme reduce the enzymatic activity and rarely used in chemical reactions. The horseradish peroxidase was immobilized on the mesoporous materials with appropriate aperture and its activity and stability was evaluated when catalyzing the nitration reaction of amines and oxidation reaction of thiourea. The optimum mesoporous material to fix the horseradish peroxidase can be obtained by mixing polyoxyethylene-polyoxypropylene-pol, yoxyethylene(P123), 1,3,5-trimethylbenzene(TMB), and tetramethoxysilane (TMOS) at a ratio of 10:1:1, whose surface area and pore volume and pore diameter calculated by BET and BJH model were 402.903m2/g, 1.084cm2/g, 1.084cm2/g respectively. The horseradish peroxidase, immobilized on the mesoporous materials, was applied for catalyzing the nitration reaction of anilines and oxidation reaction of thiourea, produced a high product yield and can be recycled. Thus, it is a strong candidate as a catalysts for oxidation reactions, to be produced at industral scale, due to its high efficiency and low cost.