Preparation of immobilized glucose oxidase wafer enzyme on calcium-bentonite modified by surfactant

Innovation in Polymer Science and Technology 2016

材料科学;化学

Widi, R.K.^1 ; Trisulo, D.C.^2 ; Budhyantoro, A.^1 ; Chrisnasari, R.^2

Department of Chemical Engineering, Surabaya University, TG Building, Jln. Raya Kalirungkut, Surabaya

60293, Indonesia^1

Department of Biology, Surabaya University, FG Building, Jln. Raya Kalirungkut, Surabaya

60293, Indonesia^2

关键词: Ca-bentonite;    Capacitance values;    Concentration ratio;    Dns methods;    Glucose oxidases (GOx);    LCR meters;    Tetramethyl ammonium hydroxide;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/223/1/012050/pdf
DOI  :  10.1088/1757-899X/223/1/012050

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Wafer glucose oxidase (GOx) enzymes was produced by addition of PAH (Poly-Allyamine Hydrochloride) polymer into immobilized GOx enzyme on modified-Tetramethylammonium Hydroxide (TMAH) 5%-calsium-bentonite. The use of surfactant molecul (TMAH) is to modify the surface properties and pore size distribution of the Ca-bentonite. These properties are very important to ensure GOx molecules can be bound on the Ca-bentonit surface to be immobilized. The addition of the polymer (PAH) is expected to lead the substrates to be adsorbed onto the enzyme. In this study, wafer enzymes were made in various concentration ratio (Ca-bentonite : PAH) which are 1:0, 1:1, 1:2 and 1:3. The effect of PAH (Poly-Allyamine Hydrochloride) polymer added with various ratios of concentrations can be shown from the capacitance value on LCR meter and enzyme activity using DNS method. The addition of the polymer (PAH) showed effect on the activity of GOx, it can be shown from the decreasing of capacitance value by increasing of PAH concentration.