The estimation of quantitative parameters of oligonucleotides immobilization on mica surface

3rd International Conference on Structural Nano Composites

材料科学;化学

Sharipov, T.I.^1 ; Bakhtizin, R.Z.^1

Department of Physical Electronics and Nanophysics, Bashkir State University, Ufa

450076, Russia^1

关键词: Calibration curves;    Degree of aggregation;    Direct imaging;    DNA chip technology;    High resolution;    Immobilization process;    Physical adsorption;    Quantitative parameters;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/195/1/012002/pdf
DOI  :  10.1088/1757-899X/195/1/012002

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Immobilization of nucleic acids on the surface of various materials is increasingly being used in research and some practical applications. Currently, the DNA chip technology is rapidly developing. The basis of the immobilization process can be both physical adsorption and chemisorption. A useful way to control the immobilization of nucleic acids on a surface is to use atomic force microscopy. It allows you to investigate the topography of the surface by its direct imaging with high resolution. Usually, to fix the DNA on the surface of mica are used cations which mediate the interaction between the mica surface and the DNA molecules. In our work we have developed a method for estimation of quantitative parameter of immobilization of oligonucleotides is their degree of aggregation depending on the fixation conditions on the surface of mica. The results on study of aggregation of oligonucleotides immobilized on mica surface will be presented. The single oligonucleotides molecules have been imaged clearly, whereas their surface areas have been calculated and calibration curve has been plotted.