Capillary electrophoresis of adenosine phosphates using boron-doped diamond electrodes

International Symposium on Current Progress in Functional Materials

Firmansyah, B.D.^1 ; Ivandini, T.A.^1 ; Gunlazuardi, J.^1

Department of Chemistry, Faculty of Mathematics, Natural Sciences Universitas Indonesia, Kampus UI Depok, Depok

16424, Indonesia^1

关键词: Adenosine monophosphate;    Boron doped diamond;    Boron doped diamond electrodes;    ELectrochemical detection;    Electrochemical detectors;    Fused-silica capillaries;    Linear calibration curve;    Phosphate buffer solutions;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/188/1/012052/pdf
DOI  :  10.1088/1757-899X/188/1/012052

来源: IOP

【 摘 要 】

A capillary electrophoresis coupled with electrochemical detection using boron-doped diamond electrode was developed for simultaneous detection of adenosine phosphates, i.e. adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP). In phosphate buffer solution pH 7, these three adenosine phosphates have similar oxidation potentials at around +0.9 V (vs. Ag/AgCl), which indicated that the oxidation occurred at the same moiety. Capillary electrophoresis, which was then performed using fused silica capillary (dia. 0.05 mm) at an applied potential of 10 KV can separate ATP, ADP and AMP with the retention times of 848 s, 1202 s, and 1439 s, respectively. Linear calibration curves with the limits of detection of 0.59 μM, 0.56 μM and 1.78 μM, respectively, can be achieved, suggested that capillary electrophoresis with electrochemical detector is promising for simultaneous detection of adenosine phosphates.