Whole blood of mammalian species in the oscillating shear field: influence of erythrocyte aggregation

4th International Conference on Competitive Materials and Technology Processes

Windberger, U.^1 ; Pöschl, Ch^1 ; Peters, S.^1 ; Huber, J.^2 ; Van Den Hoven, R.^3

Department Biomedical Research, Medical University of Vienna, Austria^1

University Clinic for Ruminants, Kremesberg, Veterinary University Vienna, Austria^2

Clinical Unit of Equine Internal Medicine, Veterinary University Vienna, Austria^3

关键词: Erythrocyte aggregation;    Frequency sweep;    Functional properties;    Mammalian species;    Oscillating shear;    Physical performance;    Small-amplitude oscillations;    Species specifics;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/175/1/012005/pdf
DOI  :  10.1088/1757-899X/175/1/012005

来源: IOP

【 摘 要 】

This is the rheologicalanalysis of mammalian blood of species with a high (horse), medium (man), and low (sheep) erythrocyte (RBC) aggregability by small amplitude oscillation technique. Amplitude and frequency sweep tests in linear mode were performed with blood from healthy adult volunteers, horses, and sheep in CSS-mode. Blood samples were hematocrit (HCT) adjusted (40%, 50%, 60%) and tested at 7°C, 22°C, and 37°C. Storage modulus (G′) increased with HCT and decreased with temperature in each species, but the gradient of this increase was species-specific. The lower dependency of G′ on the equine HCT value could be a benefit during physical performance when high numbers of RBCs are released from the spleen in the horse. In sheep, a HCT-threshold had to be overcome before elasticity of the blood sample could be measured, suggesting that the cohesive forces between RBCs, and between RBCs and plasma molecules must be very low. The frequencies for tests under quasi-staticcondition were in a narrow range around the physiologic heart rate of the species. In horse, time-dependent influences concurred at frequencies lower than 3 rad.s-1probably due to sedimentation of RBC aggregates. In conclusion, elasticity of blood depends not only on the amount of blood cells, but also on their mechanical and functional properties.