Imaging samples larger than the field of view: the SLS experience

X-Ray Microscopy Conference 2016

Oikonomidis, Ioannis Vogiatzis^1,2 ; Lovric, Goran^2,3 ; Cremona, Tiziana P.^1 ; Arcadu, Filippo^2,4 ; Patera, Alessandra^2,4 ; Schittny, Johannes C.^1 ; Stampanoni, Marco^2,4

Institute of Anatomy, University of Bern, Bern

3012, Switzerland^1

Swiss Light Source, Paul Scherrer Institute, Villigen

5234, Switzerland^2

Center for Biomedical Imaging (CIBM), EPFL, Lausanne

1015, Switzerland^3

Institute for Biomedical Engineering, University and ETH Zurich, Zurich

8092, Switzerland^4

关键词: Large amounts of data;    Micrometer ranges;    Multiple samples;    Region of interest;    Synchrotron x rays;    Temporal resolution;    Three dimensional imaging;    Volumetric data sets;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/849/1/012004/pdf
DOI  :  10.1088/1742-6596/849/1/012004

来源: IOP

【 摘 要 】

Volumetric datasets with micrometer spatial and sub-second temporal resolutions are nowadays routinely acquired using synchrotron X-ray tomographic microscopy (SRXTM). Although SRXTM technology allows the examination of multiple samples with short scan times, many specimens are larger than the field-of-view (FOV) provided by the detector. The extension of the FOV in the direction perpendicular to the rotation axis remains non-trivial. We present a method that can efficiently increase the FOV merging volumetric datasets obtained by region-of-interest tomographies in different 3D positions of the sample with a minimal amount of artefacts and with the ability to handle large amounts of data. The method has been successfully applied for the three-dimensional imaging of a small number of mouse lung acini of intact animals, where pixel sizes down to the micrometer range and short exposure times are required.