Phase-contrast tomography of sciatic nerves: image quality and experimental parameters

X-Ray Microscopy Conference 2016

Töpperwien, M.^1,2 ; Krenkel, M.^1 ; Ruhwedel, T.^3 ; Möbius, W.^2,3 ; Pacureanu, A.^4 ; Cloetens, P.^4 ; Salditt, T.^1,2

Institut für Röntgenphysik, Friedrich-Hund-Platz 1, Göttingen

37077, Germany^1

Center for Nanoscopy and Molecular Physiology of the Brain, Göttingen, Germany^2

Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Str. 3, Göttingen

37075, Germany^3

European Synchrotron Radiation Facility, Grenoble, France^4

关键词: Enhanced contrast;    Experimental parameters;    Functional structure;    Myelin sheaths;    Phase contrast tomography;    Schmidt;    Sciatic nerves;    Three dimensional (3D) structures;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/849/1/012001/pdf
DOI  :  10.1088/1742-6596/849/1/012001

来源: IOP

【 摘 要 】

We present propagation-based phase-contrast tomography of mouse sciatic nerves stained with osmium, leading to an enhanced contrast in the myelin sheath around the axons, in order to visualize the threedimensional (3D) structure of the nerve. We compare different experimental parameters and show that contrast and resolution are high enough to identify single axons in the nerve, including characteristic functional structures such as Schmidt-Lanterman incisures.