Electrophoretically deposited multiwalled carbon nanotube based amperometric genosensor for E.coli detection

India-Japan Expert Group Meeting on Biomolecular Electronics & Organic Nanotechnology for Environment Preservation

物理学;生物科学;生态环境科学

Bhardwaj, Hema^1 ; Solanki, Shipra^1 ; Sumana, Gajjala^1

Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi

110072, India^1

关键词: Chemical vapour deposition;    Concentration ranges;    Covalent immobilization;    Electrode interface;    Functionalized multi-walled carbon nanotubes;    Functionalized-MWCNT;    Fundamental characteristics;    Indium tin oxide coated glass;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/704/1/012007/pdf
DOI  :  10.1088/1742-6596/704/1/012007

来源: IOP

【 摘 要 】

This work reports on a sensitive and selective genosensor fabrication method for Escherichia coli (E.coli) detection. The functionalized multiwalled carbon nanotubes (MWCNT) synthesized via chemical vapour deposition have been deposited electrophoretically onto indium tin oxide coated glass surface and have been utilized as matrices for the covalent immobilization of E.coli specific probe oligonucleotide that was identified from the 16s rRNA coding region of the E.coli genome. This fabricated functionalized MWCNT based platform sought to provide improved fundamental characteristics to electrode interface in terms of electro-active surface area and diffusion coefficient. Electrochemical cyclic voltammetry revealed that this genosensor exhibits a linear response to complementary DNA in the concentration range of 10-7to 10-12M with a detection limit of 1×10-12M.