SINGLE BACTERIUM DETECTION USING SERS

24th International Laser Physics Workshop

Gonchukov, S.A.^1 ; Baikova, T.V.^1 ; Alushin, M.V.^1 ; Svistunova, T.S.^2 ; Minaeva, S.A.^3 ; Ionin, A.A.^4 ; Kudryashov, S.I.^1,4 ; Saraeva, I.N.^4 ; Zayarny, D.A.^4

National Research Nuclear University MEPhI, Moscow, Russia^1

Infectious Clinical Hospital No 2, Moscow, Russia^2

Institute of Laser and Information Technologies RAS, Troitsk, Russia^3

P.N. Lebedev Physical Institute RAS, Moscow, Russia^4

关键词: Biological objects;    Increasing sensitivities;    Nanostructured substrates;    Reproducibilities;    Resonance Raman scattering;    Resonant Raman Spectroscopy;    Staphylococcus aureus;    Surface enhanced Raman spectroscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/691/1/012010/pdf
DOI  :  10.1088/1742-6596/691/1/012010

来源: IOP

【 摘 要 】

This work is devoted to the study of a single Staphylococcus aureus bacterium detection using surface-enhanced Raman spectroscopy (SERS) and resonant Raman spectroscopy (RS). It was shown that SERS allows increasing sensitivity of predominantly low frequency lines connected with the vibrations of Amide, Proteins and DNA. At the same time the lines of carotenoids inherent to this kind of bacterium are well-detected due to the resonance Raman scattering mechanism. The reproducibility and stability of Raman spectra strongly depend on the characteristics of nanostructured substrate, and molecular structure and size of the tested biological object.