Quantitative phase imaging of Breast cancer cell based on SLIM

8th International Conference on Advanced Infocomm Technology

Wu, Huaqin^1 ; Li, Zhifang^1 ; Li, Hui^1 ; Wu, Shulian^1

Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China^1

关键词: Breast cancer cells;    High contrast;    Interference microscopy;    Microscopy technique;    Phase information;    Phase-contrast microscopy;    Quantitative phase imaging;    Reconstruction algorithms;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/679/1/012003/pdf
DOI  :  10.1088/1742-6596/679/1/012003

来源: IOP

【 摘 要 】

We illustrated a novel optical microscopy technique to observe cell dynamics via spatial light interference microscopy (SLIM). SLIM combines Zemike's phase contrast microscopy and Gabor's holography. When the light passes through the transparent specimens, it could render high contrast intensity and record the phase information from the object. We reconstructed the Breast cancer cell phase image by SLIM and the reconstruction algorithm. Our investigation showed that SLIM has the ability to achieve the quantitative phase imaging (QPI).