Nitric oxide-releasing polymeric nanoparticles against Trypanosoma cruzi

4th International Conference on Safe Production and Use of Nanomaterials

Seabra, A.B.^1 ; Kitice, N.A.^1 ; Pelegrino, M.T.^1 ; Lancheros, C.A.C.^2 ; Yamauchi, L.M.^2 ; Pinge-Filho, P.^3 ; Yamada-Ogatta, S.F.^2

Exact and Earth Sciences Departament, Universidade Federal de São Paulo, Rua São Nicolau 210, Diadema

CEP 09913030, SP, Brazil^1

Department of Microbiology, Universidade Estadual de Londrina, Campus Universitário, Londrina

CEP 86055-990, PR, Brazil^2

Department of Phatological Sciences, Laboratory of Immunology, Universidade Estadual de Londrina, Campus Universitário, Londrina

CEP 86055-990, PR, Brazil^3

关键词: Anti-microbial activity;    Cellular signalling;    Encapsulation efficiency;    Hydrodynamic diameter;    Mercaptosuccinic acids;    Polydispersity indices;    Polymeric nanoparticles;    Protozoan parasites;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/617/1/012020/pdf
DOI  :  10.1088/1742-6596/617/1/012020

来源: IOP

【 摘 要 】

Chagas disease, also known as American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, Trypanosoma cruzi (T. cruzi), and the disease remains a major health problem in many Latin American countries. Several papers report that the killing of the parasite is dependent on the production of nitric oxide (NO). The endogenous free radical NO is an important cellular signalling molecule that plays a key role in the defense against pathogens, including T. cruzi. As T. cruzi is able to compromise host macrophages decreasing endogenous NO production, the administration of exogenous NO donors represents an interesting strategy to combat Chagas disease. Thus, the aims of this study were to prepare and evaluate the antimicrobial activity of NO-releasing polymeric nanoparticles against T. cruzi. Biocompatible polymeric nanoparticles composed of chitosan/sodium tripolyphosphate(TPP) were prepared and used to encapsulate mercaptosuccinic acid (MSA), which is a thiol-containing molecule. Nitrosation of free thiols (SH) groups of MSA were performed by the addition of equimolar amount of sodium nitrite (NaNO2), leading to the formation of S-nitroso-MSA-containing nanoparticles. These polymeric nanoparticles act as spontaneous NO donors, with free NO release. The results show the formation of nanoparticles with average hydrodynamic diameter ranging from 270 to 500 nm, average of polydispersity index of 0.35, and encapsulation efficiency in the range of 99%. The NO release kinetics from the S-nitroso-MSA-containing nanoparticles showed sustained and controlled NO release over several hours. The microbicidal activity of S-nitroso-MSA-containing nanoparticles was evaluated by incubating NO-releasing nanoparticles (200 - 600 μg/mL) with replicative and non-infective epimastigote, and non-replicative and infective trypomastigote forms of T. cruzi. In addition, a significant decrease in the percentage of macrophage-infected (with amastigotes) and NO-releasing nanoparticle-treated cells was observed. Taken together, our results reveal a potent toxic effect of NO-releasing polymeric nanoparticles against different life cycle forms of T. cruzi, indicating that the encapsulation of the NO donor S-nitroso-MSA represents an interesting approach to combat and to prevent Chagas disease.