Polarization sensitive excitation-emission matrices for detection of colorectal tumours _ initial investigations

23rd International Laser Physics Workshop

Borisova, E.^1 ; Genova, Ts^1 ; Zhelyazkova, Al^1 ; Angelova, L.^1 ; Keremedchiev, M.^2 ; Penkov, N.^2 ; Vladimirov, B.^3 ; Semyachkina-Glushkovskaya, O.^4 ; Avramov, L.^1

Biophotonics Lab., Institute of Electronics, Bulgarian Academy of Sciences, Tsarigradsko chaussee Blvd. 72, Sofia

1784, Bulgaria^1

Surgery Departament, University Hospital Tsaritsa Yoanna-ISUL, 8 Bialo more str., Sofia

1527, Bulgaria^2

Gastroenterology Departament, University Hospital Tsaritsa Yoanna-ISUL, 8 Bialo more str., Sofia

1527, Bulgaria^3

Physiology of Human and Animals Lab., Biology Department, Saratov State University, 83 Astrakhanskaya str., Saratov, Russia^4

关键词: Autofluorescence detection;    Clinical application;    Excitation emission matrices;    Fluorescence emission;    Gastrointestinal tract;    Linear polarization;    Linear polarization mode;    Polarization sensitive;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/594/1/012031/pdf
DOI  :  10.1088/1742-6596/594/1/012031

来源: IOP

【 摘 要 】

Excitation-emission matrices were used for detection of the autofluorescence properties of surgically excised normal and cancerous mucosa of lower gastrointestinal tract tissues (colon and rectum carcinoma). Linear polarization of the excitation and emission fluorescence light was additionally applied to evaluate the influence of anisotropic fluorophores presented in the tissues investigated. Excitation applied was in the region of 280-440 nm, using step of 10 nm for the scanning, fluorescence emission was detected in the region of 300-800 nm, with scanning step of 1 nm. Excitation and emission light were investigated in parallel and perpendicular linear polarization modes respectively. These investigations are part of the concept to proof the feasibility of autofluorescence system for a real clinical application. Autofluorescence detection could make the entire diagnostics procedure more personal, patient friendly and effective and will help for further understanding of tumors nature and to improve patients' lives. In the current investigation major spectral features without and with linear polarization applied are addressed and lower GIT lesions' emission properties are evaluated.