Enzyme activity determination using ultrasound

12th Anglo-French Physical Acoustics Conference

Holmes, M.J.^1 ; Southworth, T.^1 ; Watson, N.G.^1 ; Povey, M.J.W.^1

School of Food Science and Nutrition, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, United Kingdom^1

关键词: Different substrates;    Dynamic method;    Enzyme reaction;    Rate of reaction;    Rheological measurements;    Ultra-sound velocities;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/498/1/012003/pdf
DOI  :  10.1088/1742-6596/498/1/012003

来源: IOP

【 摘 要 】

Here are presented the results of a novel approach to the measurement of enzyme reaction rates in which ultrasound velocity measurement is used. Our results show enzyme activity is observable, in the acoustic context, and that furthermore this offers the potential to estimate the rate of reaction over different substrate concentrations and temperatures. Findings are corroborated with optical microscopy and rheological measurements. Ultrasound velocity measurement can be performed without the need for aliquot extraction and offers an efficient, non-invasive and dynamic method to monitor enzyme activity.