Statistics for fluorescence photons of donor and acceptor molecules involved in non-radiative energy transfer (FRET)

16th International Youth Scientific School 'Actual Problems of Magnetic Resonance and its Applications'

Shchukina, A.L.^1 ; Osad'Ko, I.S.^1 ; Eremchev, I.Yu.^1

Institute for Spectroscopy of Russian Academy of Sciences, Troitsk 142190, Moscow, Russia^1

关键词: Donor and acceptor;    Efficiency calculations;    Electronic excitation energy;    Energy transfer efficiency;    Molecular pairs;    Multivariate approximations;    Nonradiative energy transfer;    Simulated distribution;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/478/1/012006/pdf
DOI  :  10.1088/1742-6596/478/1/012006

来源: IOP

【 摘 要 】

Fluorescence of single molecular pairs involved in Forster Resonance Energy Transfer (FRET) is considered. Cases when either a donor of the electronic excitation energy or its acceptor has a non-fluorescent "dark" state are described. The influence of such dark states on energy transfer efficiency is analytically studied. Statistics for the number of donor/acceptor photons emitted per bin time is applied for these studies. Experimental distributions for photons of single emitters with "dark" states are presented, as well as their multivariate approximations with simulated distributions. It gives an example of how emitter parameters necessary for FRET efficiency calculations (transitions to and from the dark state) can be determined from the experimental statistics.