Study on the AFM Force Spectroscopy method for elastic modulus measurement of living cells

2013 Joint IMEKO (International Measurement Confederation) TC1-TC7-TC13 Symposium: Measurement Across Physical and Behavioural Sciences

Demichelis, A.^1 ; Pavarelli, S.^1 ; Mortati, L.^1 ; Sassi, G.^1,2 ; Sassi, M.^1

Istituto Nazionale di Ricerca Metrologica I.N.Ri.M., Torino, Italy^1

Dipartimento di Scienza Dei Materiali e Ingegneria Chimica-Politecnico di Torino, Italy^2

关键词: Cell elasticity;    Elastic modulus measurements;    Force spectroscopy;    Measurement reproducibility;    Metastatic potential;    Nanoindenters;    Reference method;    Systematic effects;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/459/1/012050/pdf
DOI  :  10.1088/1742-6596/459/1/012050

来源: IOP

【 摘 要 】

The cell elasticity gives information about its pathological state and metastatic potential. The aim of this paper is to study the AFM Force Spectroscopy technique with the future goal of realizing a reference method for accurate elastic modulus measurement in the elasticity range of living cells. This biological range has not been yet explored with a metrological approach. Practical hints are given for the realization of a Sylgard elasticity scale. Systematic effects given by the sample curing thickness and nanoindenter geometry have been found with regards of the measured elastic modulus. AFM measurement reproducibility better than 20% is obtained in the entire investigated elastic modulus scale of 101-104kPa.