A method of measuring iron content in Chinese fir leaf and root tissues by using an automatic chemical analyzer

2018 1st International Conference on Environment Prevention and Pollution Control Technology

生态环境科学

Ma, Z.H.^1,2 ; Wang, P.^2,3 ; Li, N.^2,3 ; Sun, H.Y.^2,3 ; Zhang, J.^2,3 ; Cao, G.Q.^2,3 ; Chen, Y.^2,3 ; Lin, S.Z.^2,3

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou

350002, China^1

State Forestry Administration Engineering Research Center of Chinese Fir, Fuzhou

350002, China^2

College of Forestry, Fujian Agricultural and Forestry University, Fuzhou

350002, China^3

关键词: Chemical analyzers;    Concentration ranges;    Correlation coefficient;    Hydroxylamine hydrochloride;    Iron content;    Linear regression equation;    Linear relationships;    Root tissues;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/199/3/032066/pdf
DOI  :  10.1088/1755-1315/199/3/032066

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

This paper discusses a new method for determining the iron content in Chinese fir leaves and roots using a SmartChem200 automatic chemical analyzer. With a pH value of 4-5, Fe3+ was reduced to Fe2+ by hydroxylamine hydrochloride, and Fe2+ and 1,10-phenanthrolinemonohydrate generated an orange complex. The iron content was calculated based on the absorbance measured at a wavelength of 510 nm. The absorbance is directly proportional to the Fe2+ content within a certain concentration range of Fe2+. The absorbance had a good linear relationship with the Fe2+ content in the range of 0.01 mgL-1 to 5 mgL-1; the linear regression equation of the standard curve was Y=0.0632∗X-0.00002, and the correlation coefficient was 1.0000. The method is convenient and rapid, and the experimental results were reliable.