Determination colchicine content in aquadest-extracted Gloriosa superba seed from Sukoharjo and Gunung Kidul

4th International Conference on Sustainable Agriculture and Environment

农业科学;生态环境科学

Rahmawati, S.I.^1 ; Susilowati, A.^2 ; Yunus, A.^3 ; Widyastuti, Y.^4

Department of Bioscience Graduate School, Universitas Sebelas Maret, Surakarta, Central Java

57126, Indonesia^1

Department of Biology, Faculty of Mathematics and Science, Universitas Sebelas Maret, Surakarta, Central Java

57126, Indonesia^2

Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Central Java

57126, Indonesia^3

Research Center for Medicinal Plant and Traditional Drug, Tawangmangu, Indonesia^4

关键词: Alkaloid compounds;    Immature seeds;    TLC-densitometry method;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/142/1/012053/pdf
DOI  :  10.1088/1755-1315/142/1/012053

来源: IOP

【 摘 要 】

Colchicine is a toxic alkaloid compound from the Colchicum autumnale plant. Colchicine can be used in agriculture as anti-mitosis in the procurement of plants with polyploid cells. Gloriosa superba is an herb contains colchicine as a mutagen (polyploid) potential in its parts, especially in the seeds. The aims of this research were to determine colchicine content from aquadest-extracted Gloriosa superba seed obtained from Sukoharjo and Gunung Kidul. Gloriosa superba seed samples were obtained naturally in January - April 2017. Samples were divided into immature seed samples from Mulur, Sukoharjo, Central Java and old seed samples from Krakal Beach, Gunung Kidul, Yogyakarta. Extraction seeds of Gloriosa superba using the maceration method with aquadest solvent (1: 1). Determination content of colchicine extract using TLC-Densitometry method. The results indicated that colchicine content of old seed Gloriosa superba samples 37,6 μg/μl (± 6,63) higher than colchicine content of immature seed Gloriosa superba samples 12,84 μg/μl (± 2,88)