Colchicine concentration of Gloriosa superba seed at different locations and seasons

International Conference on Sustainable Agriculture for Rural Development 2018

Rahmawati, F.^1 ; Sugiyarto^2 ; Yunus, A.^3

Bioscience, Universitas Sebelas Maret, Surakarta, Indonesia^1

Faculty of Mathematic and Natural Science, Universitas Sebelas Maret, Surakarta, Indonesia^2

Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia^3

关键词: Alkaloid compounds;    Densitometry;    Environmental conditions;    Mobile phase;    Plant breeding;    Thin layers;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/250/1/012007/pdf
DOI  :  10.1088/1755-1315/250/1/012007

来源: IOP

【 摘 要 】

Colchicine is an alkaloid compound from Gloriosa superba which has potential to be developed as a mutagenic agent for plant breeding. The concentration of colchicine produced by plantis affected by different environmental conditions. This research used samples which were obtained from two locations: Sukoharjo, Central Java (collected on December 2016 until January 2017) and Gunungkidul, Yogyakarta (collected on May 2017). The colchicine concentration in G. superba seeds need to be quantified to ensure the application dose. Solvent used for extracting colchicine from G. superba seed is ethanol 70%. Thin Layer Cromatography-densitometry using Chloroform:diethylamin (9:1) as a mobile phase was used to determine colchicine concentration before validated using selectivity, accuracy, precision, and linearity parameter. The results showed that the colchicine concentration in G. superba seeds from Sukoharjo was 30,81 mg/ml and from Gunungkidul was 57,92 mg/ml when measured with densitometer on maximum wavelength 371 nm.