Biomaterial compounds and bioactivity of horseshoe crab Carsinoscorpius rotundicauda biomass harvested from the Madura Strait

2nd International Conference on Biomass: Toward Sustainable Biomass Utilization for Industrial and Energy Applications

生物科学;工业技术;能源学

Ning Asih, Eka Nurrahema^1 ; Kawaroe, Mujizat^2,3 ; Bengen, Dietriech G.^2

Post Graduate Program of Marine Science and Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor

16680, Indonesia^1

Departement of Marine Science and Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Darmaga Campus, Bogor

16680, Indonesia^2

Surfactant and Bioenergy Research Centre (SBRC), Bogor Agricultural University, Bogor

16680, Indonesia^3

关键词: Antibacterial substance;    Bacterial inhibition;    Gram-negative bacteria;    Gram-positive bacterium;    Horseshoe crab;    Legal protection;    Staphylococcus aureus;    Zone of inhibitions;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/141/1/012004/pdf
DOI  :  10.1088/1755-1315/141/1/012004

来源: IOP

【 摘 要 】

Carsinoscorpius rotundicauda or horseshoe crab biomass has great potential in pharmaceutical aspects, one of them as an antibacterial substance. Information related to the benefits of Carsinoscorpius rotundicauda biomass such as meat and blood is essential because in fact, this species is considered a pest by fishermen, a low market value and has no legal protection in Indonesia. The purpose of this study was to determine the content of biomaterial compounds of meat and bioactivity of Carsinoscorpius rotundicauda plasma on bacterial inhibition from three different stations harvested from the waters in Madura Strait. The observation of the utilization of the potential from horseshoe crab biomass ie meat and plasma was performed by measuring the content of biomaterial compound in horseshoe crab meat by HPLC method and zone of inhibition test for gram-positive and gram-negative bacteria in horseshoe crab plasma. Analysis of the relationship between the two parameters used the Principal Component Analysis. The highest content of biomaterial compounds of monoterpenoid and zoosterol is found in horseshoe crab from Bangkalan waters, namely monoterpenoid (18.33 ppm) and zoosterol (22.67 ppm), while the smallest compound content obtained in horseshoe crab from Probolinggo waters, namely monoterpenoid (13.67) ppm and zoosterol (17.33 ppm). The bioactivity of Dark Blue Plasma (BDP) and Light Blue Plasma (LBP) samples of horseshoe crab obtained around the Madura Strait has the ability to inhibit gram-positive bacteria higher than gram-negative bacteria. The total average of DBP plasma inhibitory power on Staphylococcus aureus was 10.00 mm and 10.07 mm on Bacillus, while that in LBP sample, Staphylococcus aureus was 9.11 mm and Bacillus was 9.67 mm. The high biomaterial compound content of horseshoe crab is in line with the ability of horseshoe crab plasma to inhibit Bacillus and Staphylococcus aureus.