The isolation and identification of endophytic bacteria from mangrove (Sonneratia alba) that produces gelatinase

Asean-Fen International Fisheries Symposium - 2017

Nursyam, H.^1 ; Prihanto, A.A.^1 ; Warasari, N.I.^1 ; Saadah, M.^1 ; Masrifa, R.E.^1 ; Nabila, N.A.^1 ; Istiqfarin, N.^1 ; Siddiq, I.J.^1

Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang, East Java

65145, Indonesia^1

关键词: Cell shapes;    Cultured bacteria;    Endophytic bacteria;    Gram-negative bacteria;    Identification process;    Isolation and identification;    Pseudomonas aeruginosa;    Screening process;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012056/pdf
DOI  :  10.1088/1755-1315/137/1/012056

来源: IOP

【 摘 要 】

Gelatinase is an enzyme that hydrolyze gelatin into gelatin hydrolyzate. The purpose of this study was to isolate and to identify endophytic bacteria from Sonneratia alba mangrove which able to produce gelatinase enzyme. Sonneratia alba mangroves was obtained from Bajul Mati Beach, Malang Regency. The samples in this study were, stems, and leaves. Pure cultured bacteria were investigated for its capability for producing gelatinase enzyme by using gelatin media. Best producer would further be analyzed its species using microbact system. Screening process resulted in 3 positive isolates, namely code isolate of R, B, and L. R which was isolate from root of S. alba was the best producer for gelatinase. Identification process with morphology and microbact system revelaed that A. SBM is a Gram-negative bacterium that has a basil cell shape, with a diameter colony of 2.19 mm. Based on the microbact system test carried out, the bacteria is Pseudomonas aeruginosa.