A sandwich-type optical immunosensor based on the alkaline phosphatase enzyme for Salmonella thypimurium detection

International Conference on Green Agro-industry and Bioeconomy 2017

农业科学;生物科学;经济学

Widyastuti, E.^1 ; Puspitasari Schonherr, M.F.^1 ; Masruroh, A.^1 ; Anggraeni, R.A.^1 ; Nisak, Y.K.^1 ; Mursidah, S.^2

Dept. Agricultural Product Technology, Faculty of Agricultural Technology, Brawijaya University, Indonesia^1

Dept. Agro Industrial Technology, Faculty of Agricultural Technology, Brawijaya University Veteran Street, Ketawanggede-Lowokwaru-East-Java-Malang

65145, Indonesia^2

关键词: ALkaline phosphatase;    Antibody-antigen;    Food borne disease;    Limit of quantitations;    Optical immunosensors;    Pathogenic bacterium;    Sandwich assays;    Sandwich types;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/131/1/012039/pdf
DOI  :  10.1088/1755-1315/131/1/012039

来源: IOP

【 摘 要 】

Salmonella is pathogenic bacteria that caused foodborne diseases which being called Salmonellosis. Prevalence of Salmonellosis that being caused by Salmonella thypimurium in Indonesia is quite high. However, detection of Salmonella bacteria in food still limited, complicated, and required a lot time. Sensitive optical assay for Salmonella thypimurium paper based detection has been developed by integrating sandwich assay between antibody-antigen complex and alkaline phosphatase enzyme that produce visible bluish-purple colour with presence of NBT-BCIP substrate. The results showed that Limit of Quantitation of detection is 105 CFU mL-1with detection time 15 minutes. Linearity test between Colour intensity that produced from Salmonella concentration presence on samples showed that detection has good linearity. Selectivity test exhibited excellent sensitivity with good discrimination against Escherichia coli.