Exploration of Sea Cucumbers Stichopus hermanii from Karimunjawa Islands as Production of Marine Biological Resources

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Pringgenies, Delianis^1 ; Rudiyanti, Siti^2 ; Yudiati, Ervia^1

Dept of Marine Sciences, Faculty of Fisheries and Marine Scinces Diponegoro University-Semarang, Indonesia^1

Dept of Fisheries, Faculty of Fisheries and Marine Scinces Diponegoro University-Semarang, Indonesia^2

关键词: Anti cancer;    Anti-bacteria;    Biological resources;    Chondroitin;    Chondroitin sulfates;    Glucosamine hydrochloride;    Staphylococcus aureus;    Stichopus hermanii;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012039/pdf
DOI  :  10.1088/1755-1315/116/1/012039

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

This research aim was to study the potential of Stichopus hermanii to determine the amino acid, chondroitin, and glucosamine contents, to discover its antibacterial and anti-cancer agent. The samples were rinsed prior to separation, with only the corpus being used in the study. Sea cucumber extract was then processed using HPLC to trace contents of amino acid, chondroitin, and glucosamine contents. The samples were then put into test against several strains of pathogenic bacteria by means of diffusion for any biological activity. The anti-cancer test was performed by human ovarian cancer cell line (KOC7C) method. The study showed that the extract of Stichopus hermanii has the potency to inhibit the growth of active ovarian cancer cells. The qualitative test of the sea cucumber extract showed that it is capable of suppressing the growth of several strains of pathogenic bacteria identified as Staphylococcus aureus, Escherichia coli, Vibrio voinivica, and Pseudomonas sp. HPLC results showed that the extract contained amino acid (mg/100g), the highest being Collagen (11200), followed by Glycine (3760), Glutamic Acid (3700), Aspartic Acid (2540), Alanine (2140), Proline (2050), Arginine (2050), Tyrosine (1430), Threonine (1270), Leucine (1170), Valine (1050), Serine (971), Isoleucine (816), Phenylalanine (713), Lysine (639), Methionine (383), Cystine (263) and Histidine (208). The extract also contained Chondroitin Sulfate (4200) and Glucosamine Hydrochloride (