Effect of laser radiation on the cultivation rate of the microalga Chlorella sorokiniana as a source of biofuel

All-Russian research-to-practice conference "Ecology and safety in the technosphere"

生态环境科学

Politaeva, N.^1 ; Smyatskaya, Y.^1 ; Slugin, V.^1 ; Toumi, A.^1 ; Bouabdelli, M.^1

St. Petersburg State Polytechnical University, Politeshnicheskaya, 29, St.-Petersburg, Russia^1

关键词: Biomass productions;    Chlorella sorokiniana;    Energy productions;    Extraction method;    Heat and electricity;    Microalgal biomass;    Microscopic study;    Valuable component;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/115/1/012001/pdf
DOI  :  10.1088/1755-1315/115/1/012001

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

This article studies the influence of laser radiation on the growth of micro-algal biomass of Chlorella sorokiniana. The composition of nutrient medium and the effect the laser beam (2 and 5 cm diameter, 1, 5, 10, 15 and 20 minutes exposure time) for accelerated cultivation of microalgal biomass were studied. The source of laser radiation (LR) was a helium-neon laser with a nominal output power of 1.6 mW and a wavelength of 0.63 μm. The greatest increase in biomass was observed when LR was applied to a suspension of microalga Chlorella sorokiniana with a beam of 5 cm diameter for a time of 10, 15 and 20 minutes. The results of the microscopic study of the microalga cells show a significant increase in the number of cells after an exposure to LR with a beam diameter of 5 cm in diameter. These cells were characterized by a large vacuole, a thickened lipid shell and a large accumulation of metabolites prone to agglutination. This study proposed to obtain valuable components (lipids, carotenoids, and pectin) from the obtained biomass by extraction method and to use the residual biomass formed wastes, after the extraction of valuable components, as a co-substrate for anaerobic digestion to produce biogas. The composition of biogas consists mainly of methane and carbon dioxide. Methane is recommended to be used for economic needs in supplying the whole process with heat and electricity. The carbon dioxide formed during fermentation and after combustion of methane for energy production, is planned to be used as a carbon source in the cultivation of Chlorella sorokiniana for photoautotrophic biomass production.