Establishment of a Simple and Convenient Method for Folic Acid Enzyme Chemiluminescence Immunoassay

2017 3rd International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Liu, Ting^1 ; Zeng, Ling^2 ; Yu, Zhengwei^1 ; Yang, Yanfei^1 ; Qu, Yunhuan^1

Nuclear and Radiation Safety Center, No. 54 Hongliannancun, Haidian District, Beijing, China^1

Beijing North Institute of Biological Technology, Jia No.20 Panjiamia, Fengtai District, Beijing, China^2

关键词: Chemiluminescence immunoassay;    Clinical application;    Competition model;    Correlation coefficient;    Good correlations;    Inter-assay coefficients;    Limit of detection;    Microtiter plates;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/108/2/022042/pdf
DOI  :  10.1088/1755-1315/108/2/022042

来源: IOP

【 摘 要 】

The enzyme chemiluminescence new immunoassay for folic acid (FA) was established by competition model. Add FA samples to a microtiter plate precoated with the goat anti-mouse IgG firstly, then add enzyme abled FA and FA monoclonal antibody (McAb). The values of CLIA were measured to reflect the quantity of FA. The limit of detection(LOD) of assay is 0.37ng/mL. The assay shows good correlation during 1∼30 ng/mL with correlation coefficient 0.9976. The intra- and inter-assay coefficients of variation are 4.8 % ∼ 7.3 % and 6.1 % ∼ 12.2 %, respectively. The recovery of folic acid in serum is 90.4 %∼113.2 %. Compared with determine value clinically in chemiluminescence immunoassay kit from Roche company, the correlative equation is y = 0.9689x + 0.0228, and correlation coefficient is 0.9780. Various components and kit overall show good stabilities. This method is simple and convenient, and has low LOD value. The method has overcome the shortcomings of the present references. It is easy to apply and has broad clinical application prospect. It lays an experimental foundation for the preparation of Mc Ab against folic acid and the development of domestic kit.