Evaluation Lactogenic Activity of Ethyl Acetate Fraction of Torbangun (Coleus amboinicus L.) Leaves

International Conference on Natural Products and Bioresource Science 2017

生物科学;化学

Damanik, R.M.^1 ; Kustiyah, L.^1 ; Hanafi, M.^2 ; Iwansyah, A.C.^3

Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Jl. Meranti Dramaga, Bogor

16680, Indonesia^1

Research Center for Chemistry, Indonesian Institute of Sciences, Puspiptek-Serpong, Indonesia^2

Center for Appropriate Technology Development, Indonesian Institute of Sciences, Jl. KS. Tubun No.5, Subang, West Java

41213, Indonesia^3

关键词: Bioactive compounds;    Coleus amboinicus;    Electrospray ionization mass spectrometry;    Histopathological analysis;    Phenolic compounds;    Positive ion mode;    Quadrupole-time of flights;    Ultra performance liquid chromatography;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/101/1/012007/pdf
DOI  :  10.1088/1755-1315/101/1/012007

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

This study aimed to assess the lactogenic property of ethyl acetate fraction of torbangun (Coleus amboinicus L.) leaves and to identify the compounds that responsibility as 'milk booster' using LC- MS approach. Lactagogue activity was evaluated in terms of quantity of milk produced from the rats treated with commercial milk booster (AF), ethyl acetate fraction of torbangun leaves (EA), water extraction of torbangun (AQ) and kaempferol (KP). The feed was given orally every two days and starting from Day 2 after giving birth until Day 28. The performance of milk production was measured along the experimental period by weight-suckle-weight method. The level of prolactin serum was determined by ELISA methods. Histopathological analysis of mammary gland, liver, intestines and kidney tissues was carried out. Moreover, in order to profiling and identification of compounds of ethyl acetate fraction, ultra-performance liquid chromatography quadrupole time of flight to electrospray ionization mass spectrometry (UPLC-QTOF-ESI-MS) in the positive-ion mode was performed. The ethyl acetate fraction of torbangun leaves (EA) was induced milk production about 17%, and AF 22% and KP 51% compared to the control group. Meanwhile, the EA was not significantly stimulate the synthesis of serum prolactin at Day 14 and Day 28 (p>0.05). Administration of EA did not cause any signs or symptoms of toxicity. In addition, a total of ten compounds was identified by UPLC-QTOF-ESI/MS in the ethyl acetate fraction of the leaves of C. amboinicus, mostly phenolic compounds, flavonols and some of their glycoside derivatives, such as: digiprolatone, and kaempferol-3-7-O-di-rhamnopyranoside. The present study reveals the ethyl acetate fraction of torbangun leaves and its bioactive compounds has the potency as a remedy for stimulating and improving milk production.