Pollen Characterization in Size Segregated Atmospheric Aerosol

World Multidisciplinary Earth Sciences Symposium 2017

Kolpakova, Anna^1 ; Hovorka, Jan^1 ; Klán, Miroslav^1

Air Quality Laboratory, Institute for Environmental Studies, Faculty of Science, Charles University, Benátská 2, Prague 2

128 01, Czech Republic^1

关键词: Aerodynamic diameters;    Aerosol particles;    Cascade impactors;    Czech Republic;    Impaction substrates;    Low pressure drop;    Pollen grains;    Polyurethane Foam;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/95/6/062001/pdf
DOI  :  10.1088/1755-1315/95/6/062001

来源: IOP

【 摘 要 】

The first stage of a High Volumetric Cascade Impactor - HiVol (BGI-900), used for sampling of aerosol particles larger than 10 micrometres in aerodynamic diameter, was tested for bioaerosol sampling. Low air flow-rate and low pressure-drop at the jets of the first stage and high air volume are advantageous parameters, which would favour the use of the first stage for bioaerosol sampling. The sampling went in urban, rural and background localities, Prague, Brezno and Laz respectively in the Czech Republic, in summer and autumn. Pollen was separated from the impaction substrate, polyurethane foam, into homogeneous deposit on Nylon filter. The homogeneity of the deposit varied within 4%. Representative portion of the deposit was analysed by a scanning electron microscopy - SEM. There were taken 485 SEM images from 12 samples in 3 localities. Pollen grains were identified in 295 SEM images and determined into 9 genus and 4 families. Median pollen grain concentrations/deformities were 9m-3/24%, 3m-3/18%, 8m-3/50% for Prague, Brezno and Láz localities respectively. The pollen grains of the Poaceae family were found with the highest frequency in all localities. Number of pollen increased with total aerosol mass in Prague locality only. There were also identified brochosomes, rather unique insect secretion products, in the samples from the Láz locality.