Identification of species- and tissue-specific proteins using proteomic strategy

59th International Meat Industry Conference

Chernukha, I.M.^1 ; Vostrikova, N.L.^1 ; Kovalev, L.I.^2 ; Shishkin, S.S.^2 ; Kovaleva, M.A.^2 ; Manukhin, Y.S.^1

V.M. Gorbatov All-Russian Meat Research Institute, 26, Talalikhina str, Moscow, Russia^1

Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Leninsky prospect, 33, Moscow, Russia^2

关键词: Biochemical changes;    Longissimus dorsi;    Proteomic technologies;    Sensitive technology;    Species specifics;    Tissue specifics;    Tryptic peptides;    Two dimensional electrophoresis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/85/1/012006/pdf
DOI  :  10.1088/1755-1315/85/1/012006

来源: IOP

【 摘 要 】

Proteomic technologies have proven to be very effective for detecting biochemical changes in meat products, such as changes in tissue- and species-specific proteins. In the tissues of cattle, pig, horse and camel M. Longissimus dorsi both tissue- and species specific proteins were detected using two dimensional electrophoresis. Species-specific isoforms of several muscle proteins were also identified. The identified and described proteins of cattle, pig, horse and camel skeletal muscles (including mass spectra of the tryptic peptides) were added to the national free access database "Muscle organ proteomics". This research has enabled the development of new highly sensitive technologies for meat product quality control against food fraud.