Enhancing Activity and Stability of Uricase from Lactobacillus plantarum by Zeolite immobilization

3rd International Seminar On Sciences "Sciences On Precision And Sustainable Agriculture"

Iswantini, D.^1 ; Nurhidayat, N.^2 ; Sarah^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor

16680, Indonesia^1

Division of Microbiology RandD for Biology, Indonesian Institute of Sciences, Jalan Raya Jakarta-Bogor Km 46, Cibinong Bogor

16911, Indonesia^2

关键词: Different treatments;    Disposable electrodes;    Lactobacillus plantarum;    Limit of detection;    Limit of quantifications;    Michaelis-Menten constant;    Repeated measurements;    Uric acid biosensors;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/58/1/012009/pdf
DOI  :  10.1088/1755-1315/58/1/012009

来源: IOP

【 摘 要 】

Lactobacillus plantarum has been known be able to produce uricase for uric acid biosensor. Durability and stability of L. plantarum in generating uricase enzyme was low. Hence, we tried to enhance its durability and stability by immobilizing it onto activated 250 mg zeolite at room temperature using 100 μL L.plantarum suspension and 2.87 mM uric acid, while Michaelis-Menten constant (KM) and Vmaxwere obtained at 6.7431 mM and 0.9171 μA consecutively, and the linearity range was 0.1-3.3 mM (R2= 0.9667). Limit of detection (LOD) and limit of quantification (LOQ) value of the measurement were 0.4827 mM and 1.6092 mM respectively. Biosensor stability treatment was carried out in two different treatments, using the same electrode and using disposable electrode. The disposable electrode stability showed better result based on repeated measurements, but stability was still need improvement.