A novel photoacoustic-fluorescent contrast agent for quantitative imaging of lymphatic drainage

Photoacoustics

Hyacinth Irving¹  Xun Zhou²  Neeru Gupta²  Bryan D. Koivisto³  Yeni H. Yücel⁴  Paola Luciani⁵  Kirsten Cardinell⁶  J. Carl Kumaradas⁶ 

[1] Department of Laboratory Medicine &Department of Ophthalmology and Vision Sciences, St. Michael’s Hospital, Unity Health Toronto, University of Toronto, Toronto, Ontario, Canada;Department of Physics, Faculty of Science, Ryerson University, Toronto, Ontario, Canada;Glaucoma Unit, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario, Canada;Pathobiology, St. Michael’s Hospital, University of Toronto, Toronto, Ontario, Canada;Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario, Canada;

关键词: Contrast agent;    Minimum detectable concentration;    Limit of detection;    Linear range;    Photobleaching;    Photostability;   

DOI  :  

来源: DOAJ

【 摘 要 】

In vivo near-infrared (NIR) photoacoustic imaging (PAI) studies using novel contrast agents require validation, often via fluorescence imaging. Bioconjugation of NIR dyes to proteins is a versatile platform to obtain contrast agents for specific biomedical applications. Nonfluorescent NIR dyes with higher photostability present advantages for quantitative PAI, compared to most fluorescent NIR dyes. However, they don’t provide a fluorescence signal required for fluorescence imaging. Here, we designed a hybrid PA-fluorescent contrast agent by conjugating albumin with a NIR nonfluorescent dye (QC-1) and a visible spectrum fluorescent dye, a BODIPY derivative. The new hybrid tracer QC-1/BSA/BODIPY (QBB) had a low minimum detectable concentration (2.5μM), a steep linear range (2.4–54.4 μM; slope 3.39 E -5), and high photostability. Tracer signal was measured in vivo using PAI to quantify its drainage from eye to the neck and its localization in the neck lymph node was validated with postmortem fluorescence imaging.

【 授权许可】

Unknown