Improvement of Stability and Antioxidant Activities by Using Phycocyanin - Chitosan Encapsulation Technique

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Suzery, Meiny^1 ; Hadiyanto^2 ; Majid, Dian^1 ; Setyawan, Deny^1 ; Sutanto, Heri^3

Chemistry Department, Diponegoro University, Semarang, Indonesia^1

Chemcal Engineering Department, Diponegoro University, Semarang, Indonesia^2

Physic Department, Diponegoro University, Semarang, Indonesia^3

关键词: Alkaline conditions;    Anti-oxidant activities;    Bioactive compounds;    Encapsulation process;    Mechanical stirring;    Morphological analysis;    Phycocyanin;    Physicochemical property;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012052/pdf
DOI  :  10.1088/1755-1315/55/1/012052

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Encapsulation is a coating process to improve the stability of bioactive compounds. Phycocyanin with high antioxidant activity has been encapsulated with chitosan in microcapsules form. In this study aims to determine the best conditions in the encapsulation process using the extrusion method, characterization of the physicochemical properties of the microcapsules, antioxidant activity test using DPPH, in vitro release performance and evaluate the storage stability against temperature. The results of the encapsulation process is obtained: Na-TPP is better than Na-citrate as crosslinker and chitosan content 3% as a coating with ratio of chitosan to phycocyanin ratio 1: 1. Test of antioxidant activity also showed encapsulation with chitosan content 3% has the highest antioxidant activity. Morphological analysis microcapsules were found to have compact spherical shape with diameter range 900-1000 μm. In vitro release testing showed a quick release in an acidic environment (SGF) for 2 hours and slowly release under alkaline conditions (SIF) for 8 hours under mechanical stirring at 37°C. Phycocyanin much more stable against temperature during storage in microcapsules.