Inhibition of α-glucosidase activity by ethanolic extract of Melia azedarach L. leaves

Workshop and International Seminar on Science of Complex Natural Systems

Sulistiyani^1,2 ; Safithri, Mega^1 ; Sari, Yoana Puspita^1

Departement of Biochemistry, Bogor Agricultural University, Jl. Agathis Dramaga, Bogor, Indonesia^1

Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No.3, Bogor, Indonesia^2

关键词: Alpha glucosidase;    Economic management;    Ethanolic extracts;    Glucosidase inhibitors;    Inhibitory activity;    Melia azedarach;    Natural products;    Potential inhibitors;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/31/1/012025/pdf
DOI  :  10.1088/1755-1315/31/1/012025

来源: IOP

【 摘 要 】

Development of α-glucosidase inhibitor derived from natural products is an opportunity for a more economic management of diabetes prevention. The objective of this study was to test the activity of α-glucosidase with or without potential inhibitor compounds. By in vitro method, α-glucosidase hydrolizes p-nitrophenyl-α-D-glucopiranoside to glucose and the yellow of p-nitrophenol which can be determined with spectrophotometry at 400 nm. The ability of ethanolic leaf extract of Melia azedarach L. as a-glucosidase inhibitor was compared with that of commercial acarbose (Glucobay®). Acarbose showed strong inhibitory activity against a-glucosidase with IC50values of 2.154 g/mL. The crude ethanolic leaf extract of M. azedarach, however, showed less inhibitory activity with IC50value of 3, 444.114 g/mL. Total phenolics of M. azedarach leaves EtOH extract showed 17.94 g GAE/mg extract and flavonoids total compound of 9.55 g QE/mg extract. Based on the published wide range of IC50values of extracts reported as a-glucosidase inhibitor which were between 10, 000 ppm-0.66 ppm, our result suggests that extract of M.azedarach leaves is potential candidate for development of anti-hyperglycemic formulation.