Workshop and International Seminar on Science of Complex Natural Systems | |
Inhibition of α-glucosidase activity by ethanolic extract of Melia azedarach L. leaves | |
Sulistiyani^1,2 ; Safithri, Mega^1 ; Sari, Yoana Puspita^1 | |
Departement of Biochemistry, Bogor Agricultural University, Jl. Agathis Dramaga, Bogor, Indonesia^1 | |
Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No.3, Bogor, Indonesia^2 | |
关键词: Alpha glucosidase; Economic management; Ethanolic extracts; Glucosidase inhibitors; Inhibitory activity; Melia azedarach; Natural products; Potential inhibitors; | |
Others : https://iopscience.iop.org/article/10.1088/1755-1315/31/1/012025/pdf DOI : 10.1088/1755-1315/31/1/012025 |
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来源: IOP | |
【 摘 要 】
Development of α-glucosidase inhibitor derived from natural products is an opportunity for a more economic management of diabetes prevention. The objective of this study was to test the activity of α-glucosidase with or without potential inhibitor compounds. By in vitro method, α-glucosidase hydrolizes p-nitrophenyl-α-D-glucopiranoside to glucose and the yellow of p-nitrophenol which can be determined with spectrophotometry at 400 nm. The ability of ethanolic leaf extract of Melia azedarach L. as a-glucosidase inhibitor was compared with that of commercial acarbose (Glucobay®). Acarbose showed strong inhibitory activity against a-glucosidase with IC50values of 2.154 g/mL. The crude ethanolic leaf extract of M. azedarach, however, showed less inhibitory activity with IC50value of 3, 444.114 g/mL. Total phenolics of M. azedarach leaves EtOH extract showed 17.94 g GAE/mg extract and flavonoids total compound of 9.55 g QE/mg extract. Based on the published wide range of IC50values of extracts reported as a-glucosidase inhibitor which were between 10, 000 ppm-0.66 ppm, our result suggests that extract of M.azedarach leaves is potential candidate for development of anti-hyperglycemic formulation.
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