Antioxidant activity evaluation of aqueous extracts of chickpea and lentil seedlings

International Scientific Conference «AGRITECH-2019: Agribusiness, Environmental Engineering and Biotechnologies»

农业科学;生态环境科学;生物科学

Gubanenko, G.A.^1 ; Zykova, I.D.^1 ; Naimushina, L.V.^1 ; Rechkina, E.A.^2 ; Mayurnikova, L.A.^3

Siberian Federal University, Svobodny ave. 79, Krasnoyarsk

660041, Russia^1

Krasnoyarsk State Agrarian University, Mira str. 90, Krasnoyarsk

660049, Russia^2

Kemerovo State University, Krasnaya str. 6, Kemerovo

650000, Russia^3

关键词: Anti-oxidant activities;    Aqueous extracts;    Biologically active compounds;    Intermediate product;    Intermediate stage;    Leguminous crops;    Model reactions;    Reducing sugars;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/315/5/052015/pdf
DOI  :  10.1088/1755-1315/315/5/052015

来源: IOP

【 摘 要 】

The aim of the study was to explore the antioxidant activity of aqueous extracts of leguminous crop seedlings - chickpea and lentils. For the study of antioxidant activity, an in vitro method of autooxidation of adrenaline in an alkaline medium was used. The process of adrenaline autooxidation was investigated as a model reaction of quinoid oxidation, proceeding through a series of intermediate stages with the formation of the final product of adrenochrome. The accumulation of the adrenaline autooxidation intermediate product - adrenalinquinone - in an aqueous-alkaline medium and in the presence of aqueous chickpea or lentil extracts was recorded using a UV 1700 spectrophotometer (Shimadzu) at a wavelength of 348 nm. It was revealed that aqueous extracts of legumes exhibit antioxidant activity. Inhibition of adrenaline autooxylation with a selected exposure time of 5 minutes in the presence of aqueous extracts of lentils was 25%, and of chickpea - 16%. The interrelation of the manifestation of the antioxidant activity of seedlings and the content of biologically active compounds with reducing properties (reducing sugars, flavonoids, vitamin C and tannins) was revealed.