Antibacterial activity of the symbiotic bacteria of green algae Caulerpa racemosa from Pulau Lima Indonesia

3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation

生态环境科学

Sujuliyani^1 ; Thaib, E.A.^2 ; Indriati, N.^3 ; Liananda, M.^1

Study Program of Fisheries Processing Technology, Jakarta Fisheries University, Indonesia^1

Study Program of Aquaculture, Jakarta Fisheries University, Indonesia^2

Marine and Fisheries Biotechnology and Product Processing Research Center, Jakarta, Indonesia^3

关键词: Anti-bacterial activity;    antibacterial;    Bioactive compounds;    Caulerpa racemosa;    Gram-negative bacteria;    ITS2 markers;    Pathogenic bacterium;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/278/1/012075/pdf
DOI  :  10.1088/1755-1315/278/1/012075

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The use of symbionts is the most efficient way to obtain antibacterial bioactive compounds without the extraction of the host plant. Caulerpa racemosa or sea grapes are known to have symbiotic bacteria. This study was aimed to examine the antibacterial activity of symbiotic bacteria of C. racemosa. The research obtained two isolates from the inside of algae that formed an obstruent zone and of antagonist activity against Staphylococcus aureus and Salmonella typhi bacteria. Further analysis of the isolate coded CaD51 by paper disc diffusion showed that the bacteria produced an obstruent zone by 5.08 mm against S. aureus and had 50% Minimum Inhibitory Concertration (MIC) on both pathogenic bacteria. The bacteria reached their optimal growth rate at the 16th to 20th hour. The CaD51 were identified as gram negative bacteria, coccus, acid intolerant, not producing spores, not motile, and anaerobic facultative. Based on these key identification properties, the isolate CaD51 belongs to the genus Neisseria.